Denaturation of nucleic acids induced by intercalating agents. Biochemical and biophysical properties of acridine orange-DNA complexes

Journal of Biomolecular Structure & Dynamics
J Kapuscinski, Z Darzynkiewicz

Abstract

At high binding densities acridine orange (AO) forms complexes with ds DNA which are insoluble in aqueous media. These complexes are characterized by high red- and minimal green-luminescence, 1:1 (dye/P) stoichiometry and resemble complexes of AO with ss nucleic acids. Formation of these complexes can be conveniently monitored by light scatter measurements. Light scattering properties of these complexes are believed to result from the condensation of nucleic acids induced by the cationic, intercalating ligands. The spectral and thermodynamic data provide evidence that AO (and other intercalating agents) induces denaturation of ds nucleic acids; the driving force of the denaturation is high affinity and cooperativity of binding of these ligands to ss nucleic acids. The denaturing effects of AO, adriamycin and ellipticine were confirmed by biochemical studies on accessibility of DNA bases (in complexes with these ligands) to the external probes. The denaturing properties of AO vary depending on the primary structure (sugar- and base-composition) of nucleic acids.

References

Oct 1, 1975·Experimental Cell Research·Z DarzynkiewiczM R Melamed
Jan 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Z DarzynkiewiczM R Melamed
Jan 1, 1978·Progress in Biophysics and Molecular Biology·H Porumb
May 29, 1979·Biochemistry·R W Wilson, V A Bloomfield
Nov 4, 1976·Journal of Molecular Biology·M T RecordP De Haseth
May 1, 1975·Cancer· Min K-WB Halpert
Jul 10, 1974·Biochemical and Biophysical Research Communications·Z DarzynkiewiczM Melamed
Apr 30, 1971·European Journal of Biochemistry·B Oberg
May 20, 1970·Journal of the American Chemical Society·R W ArmstrongU P Strauss
Jan 1, 1982·Cytometry·J KapuscinskiM R Melamed
Oct 1, 1983·Experimental Cell Research·Z DarzynkiewiczM R Melamed
Jan 1, 1983·Progress in Biophysics and Molecular Biology·S Neidle, H M Berman
Dec 25, 1980·Journal of Molecular Biology·J Widom, R L Baldwin
Jul 31, 1984·Biochemical and Biophysical Research Communications·S ChandrasekaranW D Wilson
Nov 11, 1983·Nucleic Acids Research·J Kapuscinski, Z Darzynkiewicz
Sep 1, 1982·Cytometry·M IngramA Castro
Jan 1, 1981·Annual Review of Biochemistry·M T RecordL Unger
Jan 1, 1956·Proceedings of the Society for Experimental Biology and Medicine·D F CLAUSEN, N LIFSON
Feb 1, 1961·Journal of Molecular Biology·L S LERMAN
Aug 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·V N IYER, W SZYBALSKI
Jul 1, 1959·Proceedings of the National Academy of Sciences of the United States of America·D F Bradley, M K Wolf

❮ Previous
Next ❯

Citations

Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J Kapuscinski, Z Darzynkiewicz
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Kapuscinski, Z Darzynkiewicz
Apr 11, 1995·Nucleic Acids Research·H S Rye, A N Glazer
Sep 1, 1995·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·J Kapuscinski
Aug 21, 2010·Journal of Nucleic Acids·Gaëlle Lenglet, Marie-Hélène David-Cordonnier
Dec 15, 1985·Biochemical Pharmacology·J Kapuscinski, Z Darzynkiewicz
Aug 14, 1992·Biochemical and Biophysical Research Communications·G DodinS Nocentini
Mar 1, 1987·Cytometry·J Kapuscinski, Z Darzynkiewicz
Mar 1, 1987·Cytometry·Z DarzynkiewiczH A Crissman
Aug 1, 1987·Journal of Biomolecular Structure & Dynamics·J Kapuscinski, Z Darzynkiewicz
Feb 1, 1992·Journal of Biomolecular Structure & Dynamics·E Morier-TeissierE Delain

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
J Kapuscinski, Z Darzynkiewicz
The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
J Kapuscinski
© 2021 Meta ULC. All rights reserved