Denatured collagen modulates the phenotype of normal and wounded human skin equivalents.

The Journal of Investigative Dermatology
Christophe EglesJonathan A Garlick

Abstract

Epithelial-mesenchymal interactions are known to play an important role in modulating homeostasis and repair. However, it remains unclear how the composition of the extracellular matrix may regulate the ability of dermal fibroblasts to engage in such cross talk. To address this, we studied how fibroblast phenotype was linked to the behavior of normal and wounded human skin equivalents (HSE) by comparing human dermal fibroblasts (HDF) incorporated into the three-dimensional tissues to those extensively cultivated in two-dimensional (2D) monolayer culture on denatured collagen (DC) matrix, native collagen, or tissue culture plastic before incorporation into HSEs. We first established that prolonged passage and growth of HDF on DC increased their migratory potential in a 2D monolayer culture. When HDF variants were grown in HSEs, we found that extended passage on DC and incorporation of DC directly into the collagen gel enhanced proliferation of both HDF and basal keratinocytes in HSEs. By adapting HSEs to study wound reepithelialization, we found that the extended passage of HDF on DC accelerated the rate of wound healing by 38%. Thus, extensive ex vivo expansion on DC was able to modify the phenotype of skin fibroblasts by augme...Continue Reading

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Grinnell, D Minter
Nov 1, 1992·The Journal of Dermatology·J A Garlick, L B Taichman
May 1, 1982·Mechanisms of Ageing and Development·A L Muggleton-HarrisR L Burghoff
Sep 1, 1994·Clinical Immunology and Immunopathology·K M FriesR P Phipps
Oct 1, 1994·The Journal of Investigative Dermatology·J A Garlick, L B Taichman
Jul 1, 1993·The American Journal of the Medical Sciences·R A Clark
Sep 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Har-el, M L Tanzer
Mar 1, 1996·Journal of Dental Research·J A GarlickL B Taichman
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
Aug 30, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·J Heino
Mar 20, 2002·Nature Reviews. Cancer·M J Bissell, D Radisky
Oct 24, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Vladimir Volloch, David Kaplan
Jun 6, 2003·The Journal of Investigative Dermatology·Frank AndrianiJonathan A Garlick
Feb 21, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Pornprom MuangmanNicole S Gibran
Apr 17, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Mohammad Ali-BaharAziz Ghahary
Jun 3, 2004·Journal of Biomedical Materials Research. Part a·Leah C AbrahamDavid L Kaplan
May 4, 2005·The International Journal of Lower Extremity Wounds·David T Rovee
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Oct 31, 2006·The Journal of Investigative Dermatology. Symposium Proceedings·Beate EckesThomas Krieg
Feb 6, 2007·Experimental Cell Research·Leah C AbrahamDavid L Kaplan
Apr 17, 2007·The Journal of Investigative Dermatology·Sabine WernerHans Smola
Apr 17, 2007·The Journal of Investigative Dermatology·Richard A F ClarkMarcia G Tonnesen

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Citations

May 22, 2010·Tissue Engineering. Part a·Balajikarthick SubramanianDavid L Kaplan
Jul 13, 2011·The Journal of Clinical Investigation·Ron C J SchackmannPatrick W B Derksen
Feb 23, 2011·Stem Cell Research & Therapy·Yulia ShamisJonathan A Garlick
Jan 8, 2010·Biomaterials·Anna V TaubenbergerDietmar W Hutmacher
Sep 8, 2015·European Journal of Cell Biology·Gopu SriramMei Bigliardi-Qi
Jan 3, 2013·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Macrene Alexiades-ArmenakasBasil M Hantash
Nov 6, 2009·Nature Reviews. Urology·Karen J Aitken, Darius J Bägli
Mar 25, 2014·Expert Review of Medical Devices·Luis M DelgadoDimitrios I Zeugolis
Apr 29, 2015·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Macrene Alexiades, Dany Berube

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