Denatured thiolated collagen. II. Cross-linking by oxidation

Biomaterials
F L Nicolas, C H Gagnieu

Abstract

We have recently described a new method for the thiolation of denatured collagen, which allows precise amounts of SH groups to be attached onto the protein backbone. The oxidation of denatured thiolated collagen produces disulphide cross-linking. The cross-linking of these products has been studied, optimized and compared to the cross-linking of native and denatured collagen with 0.5% aqueous glutaraldehyde. Films have been prepared and their tensile mechanical properties and biodegradation rates with trypsin and collagenase have been evaluated. Our results indicate that the cross-linking in oxidized thiolated collagen depends on the number of the disulphide bridges formed and on their intermolecular versus intramolecular repartition. Since the number of disulphide bridges can be controlled by the level of thiol in the denatured collagen and by the oxidation procedure, it is possible to control the mechanical properties and the biodegradation rates of these new materials. Under optimized conditions, oxidized denatured thiolated collagen films are more resistant and rigid than glutaraldehyde-cross-linked collagen films Cross-linked thiolated collagen materials are also more resistant to collagenase degradation. However, because ...Continue Reading

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Citations

Apr 12, 2002·Journal of Biomedical Materials Research·T MatsumotoJ Takahashi
Feb 7, 2002·Biomaterials·Cécile F Rousseau, Christian H Gagnieu
Jun 9, 2001·International Journal of Pharmaceutics·C H LeeY Lee
Dec 19, 2009·Regenerative Medicine·Swathi Ravi, Elliot L Chaikof
Nov 15, 2000·Journal of Biomedical Materials Research·V Charulatha, A Rajaram
Aug 27, 2005·Macromolecular Bioscience·Jason HabermehlDiego Mantovani
Jan 27, 2019·Journal of Biomedical Materials Research. Part a·Samaneh GhazanfariAli Khademhosseini
Dec 22, 2009·Chemical Communications : Chem Comm·R VijayaraghavanP K Sehgal
May 5, 2007·Macromolecular Bioscience·Frédéric CouetDiego Mantovani
May 18, 2020·International Journal of Molecular Sciences·Daniel Wee Yee ToongYingying Huang
Jul 18, 2015·International Journal of Molecular Sciences·Juan LiuArndt F Schilling
May 12, 2009·Vascular·Swathi RaviElliot L Chaikof

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