Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community.

Applied and Environmental Microbiology
M J FerrisD M Ward

Abstract

Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene segments was used to profile microbial populations inhabiting different temperature regions in the microbial mat community of Octopus Spring, Yellowstone National Park. DGGE allowed a rapid evaluation of the distributions of amplifiable sequence types. Profiles were essentially identical within regions of the mat defined by one temperature range but varied between sites with different temperature ranges. Individual DGGE bands were sequenced, and the sequences were compared with those previously obtained from the mat by cloning and from cultivated Octopus Spring isolates. Two known cyanobacterial populations and one known green nonsulfur bacterium-like population were detected by DGGE, as were many new cyanobacterial and green nonsulfur and green sulfur bacterium-like populations and a novel bacterial population of uncertain phylogenetic affiliation. The distributions of several cyanobacterial populations compared favorably with results obtained previously by oligonucleotide probe analyses and suggest that adaptation to temperature has occurred among cyanobacteria which are phylogenetically very similar.

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Citations

Jul 6, 2000·Applied and Environmental Microbiology·G M ColoresW P Inskeep
Jan 3, 2001·Applied and Environmental Microbiology·A G SpeksnijderH J Laanbroek
Aug 30, 2001·Applied and Environmental Microbiology·U NübelD M Ward
Oct 27, 2001·Applied and Environmental Microbiology·T HoshinoY Inamori
Mar 7, 2003·Applied and Environmental Microbiology·Y Meriah Arias, Bradley M Tebo
Jun 23, 2005·Extremophiles : Life Under Extreme Conditions·Jing HongmeiStephen B Pointing
Apr 7, 2005·Applied and Environmental Microbiology·Yossi TalHarold J Schreier
Apr 7, 2006·Applied and Environmental Microbiology·Kirsti M RitalahtiFrank E Löffler
Jan 18, 2007·Environmental Microbiology·Guus RoeselersGerard Muyzer
Jul 31, 2007·Applied and Environmental Microbiology·Olga SánchezCarlos Pedrós-Alió
Jan 18, 2007·Environmental Microbiology·Andrew BissettJohn P Bowman
Feb 20, 2007·Applied and Environmental Microbiology·Corinne R LehrTimothy R McDermott
Sep 11, 2007·Applied and Environmental Microbiology·Jinjun KanFeng Chen
Nov 1, 2008·The Journal of Microbiology·Young-Do NamJin-Woo Bae
Jun 3, 2009·Journal of Applied Microbiology·R LaforgueM Bouix
Jul 14, 2010·Applied and Environmental Microbiology·Heidi M LuterNicole S Webster
Jul 10, 2012·Applied and Environmental Microbiology·Kathleen M MorrowMark R Liles
Feb 27, 2013·Environmental Technology·Pao-Wen Grace LiuMing-Zhi Wang
Dec 17, 2014·International Journal of Phytoremediation·Karis N NelsonRaina M Maier
Mar 5, 2015·Environmental Technology·Graziella Patrício Pereira GarciaJuliana Calabria de Araujo
Nov 6, 2018·Water Science and Technology : a Journal of the International Association on Water Pollution Research·L S AzevedoC A L Chernicharo
Oct 3, 2020·Microbiome·Alexandra S TauzinGabrielle Potocki-Veronese

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