Denaturing polyacrylamide gel electrophoresis

Current Protocols in Nucleic Acid Chemistry
L M Albright, B E Slatko

Abstract

Thin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (<500 nucleotides) single-stranded fragments of DNA or RNA that differ in length by as little as one nucleotide. Such gels are uniquely suited for nucleic acid sequence analysis, which is required, for instance, for all footprinting protocols. Thicker gels are often used to purify oligonucleotides. This appendix describes the pouring, running, and processing of a typical sequencing gel, which is 40 cm long with a uniform thickness of 0.4 mm, containing 7 M urea and 4% to 8% acrylamide.

References


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Citations

Oct 30, 2009·Journal of Visualized Experiments : JoVE·Heike SummerPeter Dröge
Oct 16, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Amberlyn M PetersonJennifer M Heemstra
Mar 13, 2019·Current Protocols in Nucleic Acid Chemistry·Ken YamadaFumi Nagatsugi
Jun 2, 2016·Current Protocols in Nucleic Acid Chemistry·Hiroto Fujita, Masayasu Kuwahara
Jun 12, 2012·Current Protocols in Pharmacology·John L NitissMargarita Mishina
Jun 20, 2017·Current Protocols in Nucleic Acid Chemistry·Oleksandr PlashkevychJyoti Chattopadhyaya
Sep 19, 2019·Current Protocols in Nucleic Acid Chemistry·Marleen RendersPiet Herdewijn
May 17, 2002·The New England Journal of Medicine·Neng YuEdmond J Yunis
Aug 11, 2019·Nature Communications·Saskia GresselPatrick Cramer
Oct 5, 2021·Current Protocols·John L NitissNancy Maizels

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