Dendritic Cell Activity Driven by Recombinant Mycobacterium bovis BCG Producing Human IL-18, in Healthy BCG Vaccinated Adults

Journal of Immunology Research
Piotr SzpakowskiMagdalena Kowalewicz-Kulbat

Abstract

Tuberculosis remains an enormous global burden, despite wide vaccination coverage with the Bacillus Calmette-Guérin (BCG), the only vaccine available against this disease, indicating that BCG-driven immunity is insufficient to protect the human population against tuberculosis. In this study we constructed recombinant BCG producing human IL-18 (rBCGhIL-18) and investigated whether human IL-18 produced by rBCGhIL-18 modulates DC functions and enhances Th1 responses to mycobacterial antigens in humans. We found that the costimulatory CD86 and CD80 molecules were significantly upregulated on rBCGhIL-18-infected DCs, whereas the stimulation of DCs with nonrecombinant BCG was less effective. In contrast, both BCG strains decreased the DC-SIGN expression on human DCs. The rBCGhIL-18 increased IL-23, IL-10, and IP-10 production by DCs to a greater extent than nonrecombinant BCG. In a coculture system of CD4(+) T cells and loaded DCs, rBCGhIL-18 favoured strong IFN-γ but also IL-10 production by naive T cells but not by memory T cells. This was much less the case for nonrecombinant BCG. Thus the expression of IL-18 by recombinant BCG increases IL-23, IP-10, and IL-10 expression by human DCs and enhances their ability to induce IFN-γ and...Continue Reading

References

Feb 1, 1995·Journal of Bacteriology·L KremerC Locht
Sep 1, 1995·Molecular Microbiology·L KremerC Locht
Oct 1, 1998·European Journal of Immunology·F SallustoA Lanzavecchia
Mar 15, 2002·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Franck BietLaurent Kremer
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer H DufourAndrew D Luster
Dec 11, 2002·Scandinavian Journal of Immunology·C-H SongE-K Jo
Jan 8, 2003·The Journal of Experimental Medicine·Teunis B H GeijtenbeekYvette Van Kooyk
Dec 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ralf GutzmerThomas Werfel
Nov 19, 2004·Current Opinion in Hematology·Pavan Reddy
Dec 20, 2005·The Journal of Pathology·Timo Ulrichs, Stefan H E Kaufmann
May 4, 2006·The Journal of Clinical Investigation·David YenDonna Rennick
Jul 22, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael J RobertsonMohammed M Dar
Jun 4, 2008·Folia Histochemica Et Cytobiologica·Magdalena Kowalewicz-KulbatWiesława Rudnicka
Jun 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael J RobertsonMohammed M Dar
Sep 13, 2008·American Journal of Respiratory Cell and Molecular Biology·Richard F SilverJoseph P Sypek
Dec 2, 2009·European Journal of Immunology·Bianca E SchneiderUlrich E Schaible
Apr 9, 2010·Journal of Innate Immunity·Cory M RobinsonGerard J Nau
May 12, 2010·European Journal of Immunology·Amy N SieveRance E Berg
May 19, 2010·Expert Review of Respiratory Medicine·Morten Ruhwald, Pernille Ravn
Jul 6, 2010·European Journal of Immunology·Lisa M ConnorJoanna R Kirman
Jan 5, 2011·Protein & Cell·Maria V CarrollDaniel A Mitchell
Apr 1, 2011·Mucosal Immunology·P S RedfordA O'Garra
Jul 30, 2011·The Lancet Infectious Diseases·Stefan H E Kaufmann
Aug 10, 2011·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Alagar Manickam, Muthukumaran Sivanandham
Sep 7, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Helen McShane
Sep 6, 2012·European Journal of Immunology·Andrew L CroxfordBurkhard Becher
Sep 11, 2012·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Ji Young HongYoung Ae Kang
Feb 13, 2013·Clinical & Developmental Immunology·Magdalena DruszczynskaWieslawa Rudnicka
Mar 23, 2013·Annual Review of Immunology·Anne O'GarraMatthew P R Berry

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Citations

May 24, 2017·Expert Review of Vaccines·Magdalena Kowalewicz-Kulbat, Camille Locht
Nov 30, 2019·Journal of Translational Medicine·Marina DegoricijaJanos Terzic
May 8, 2021·Frontiers in Immunology·Charles Yin, Bryan Heit
Aug 22, 2021·Vaccine·Magdalena Kowalewicz-Kulbat, Camille Locht

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Methods Mentioned

BETA
reverse transcription-PCR
PCR
flow cytometry
FCS
FACS
ELISA

Software Mentioned

FlowJo

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