Dendritic cell-based immunization induces Coccidioides Ag2/PRA-specific immune response

Vaccine
Shanjana AwasthiNegar Rahman

Abstract

Valley Fever, or coccidioidomycosis, is caused by a soil-borne, highly virulent fungal pathogen, Coccidioides spp. Infection with Coccidioides can be life-threatening. Since an effective treatment is not available and the T cell-mediated immune response is protective, vaccine development is of interest. In this study, a primary dendritic cell (DC)-vaccine was evaluated for its ability to stimulate Coccidioides antigen-specific immune response in an extremely susceptible BALB/c mouse model. The DC-vaccine (Ag2-DC) was prepared by non-virally transfecting the primary bone marrow-derived DCs with a plasmid DNA encoding Ag2/PRA (protective epitope of Coccidioides). Mice were intranasally immunized with Ag2-DC on days 2 and 10. Immunized mice were necropsied on days 8, 32, and 44. Major organs and blood samples were harvested. The most common indicators of injury (protein, lactate, and albumin), Ag/PRA-specific cytokine-secreting cells, and IgG and its isotypes were determined by biochemical and immunologic assays, respectively. No signs of sickness were noted. Similarly, no significant changes were observed in the levels of total lung protein, lactate, and albumin, in immunized mice compared with healthy control mice. Interferon (I...Continue Reading

Citations

Oct 2, 2019·Frontiers in Immunology·Marley C Caballero Van DykeBridget M Barker
Oct 18, 2019·Clinical Microbiology Reviews·Fariba M DonovanJohn N Galgiani
Jun 25, 2020·BMC Pulmonary Medicine·Shanjana AwasthiStanley Kosanke
Jul 30, 2020·Journal of Fungi·Leandro B R Da SilvaJoshua D Nosanchuk
Jan 12, 2021·Frontiers in Cellular and Infection Microbiology·Juan David Puerta-AriasÁngel González
Dec 3, 2020·Frontiers in Cellular and Infection Microbiology·Anh L Diep, Katrina K Hoyer
Jun 3, 2021·Journal of Fungi·Rebecca A WardJatin M Vyas

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