Dendritic cell-based immunogens for B-cell chronic lymphocytic leukemia

Cancer Letters
Thomas AllgeierRalph Mocikat

Abstract

Hybrids generated from tumor cells and dendritic cells (DC) have been proposed as tools for treating malignant disease. Here, we study the underlying principles and the feasibility for the adjuvant therapy of human B cell chronic-lymphocytic leukemia (B-CLL). CLL cells and allogeneic DC were only mixed or additionally fused. Using a combination of FACS and fluorescence microscopic analyses, we show that DC-CLL hybrids can be successfully generated. However, fusion frequencies have to be critically evaluated because the number of fused cells is overestimated when based on FACS analyses alone. The capability of activating patients' PBMC was examined by measuring cytokine secretion in co-culture assays. We made a systematic comparison of the immunostimulatory capacities of different stimulator cell populations, including DC-CLL fusion samples, unfused mixtures of DC and CLL cells as well as DC or tumor cells alone. Surprisingly, even unfused mixtures had a pronounced tumor-directed immunostimulatory effect. This could be explained by the capture of antigens from surrounding leukemia cells by DC during co-cultivation. Although fusion frequencies were low, PBMC stimulation was significantly more effective when the mixtures were subj...Continue Reading

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Citations

May 5, 2011·Journal of Biomedicine & Biotechnology·Shigeo KoidoHisao Tajiri
Apr 13, 2010·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yan GeXue-Guang Zhang
Nov 5, 2010·Clinical & Developmental Immunology·Shigeo KoidoHisao Tajiri
Mar 7, 2007·Expert Review of Anticancer Therapy·Anita SchmittMichael Schmitt

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