Dendritic cell-mediated stimulation of the in vitro lymphocyte response to Aspergillus

Bone Marrow Transplantation
M GrazziuttiC A Savary

Abstract

Lymphocytes play a major role in host defense against Aspergillus, but little is known about the contribution of dendritic cells (DC) to antifungal immunity in humans. We have observed that DC derived from normal volunteers phagocytose heat-killed A. fumigatus conidia. Following 24 h of exposure to the fungus, DC displayed an increase in the mean fluorescence intensity of HLA-DR, CD80, and CD86, and an increase in the percentage of CD54(+) cells. These DC also displayed increased production of IL-12. DC derived from CD34(+) progenitors or monocytes stimulated autologous lymphocytes to proliferate and produce high levels of interferon-gamma, but not interleukin-10, in response to fungal antigen. DC generated from CD34(+) progenitors collected prior to autologous or allogeneic stem cell transplantation also partially restored the in vitro antifungal proliferative response of lymphocytes obtained from patients 1 month after transplantation. These results suggest that DC are important to host-response to A. fumigatus, and that ex vivo-generated DC might be useful in restoring or enhancing the antifungal immunity after hematopoietic stem cell transplantation.

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Citations

Oct 6, 2007·Immunologic Research·Ryan A Langlois, Kevin L Legge
Nov 7, 2002·Trends in Microbiology·Luigina RomaniPaolo Puccetti
Aug 6, 2002·Current Opinion in Microbiology·Michael K Mansour, Stuart M Levitz
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Nov 6, 2004·Blood Cells, Molecules & Diseases·Katia PerruccioLuigina Romani
Nov 10, 2004·International Journal of Medical Microbiology : IJMM·Heidrun Moll
Apr 25, 2007·FEMS Immunology and Medical Microbiology·Jorge Pereira da SilvaClaudio Guedes Salgado
Dec 9, 2003·Nature Reviews. Immunology·Luigina Romani
Sep 1, 2006·Medical Mycology·Teresa ZelanteLuigina Romani
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Angela BacciLuigina Romani
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Silvia BozzaLuigina Romani
Oct 8, 2016·Journal of Applied Toxicology : JAT·Akiko HondaHirohisa Takano
Jan 24, 2018·Frontiers in Immunology·Pappanaicken R KumaresanDimitrios P Kontoyiannis
Jun 7, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shaunna M HustonChristopher H Mody
Sep 5, 2018·Frontiers in Cellular and Infection Microbiology·Esther WeissJuergen Loeffler

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