PMID: 11315508Apr 24, 2001Paper

Dendritic-cell-peptide immunization provides immunoprotection against bcr-abl-positive leukemia in mice

Cancer Immunology, Immunotherapy : CII
L HeEmmanuel Katsanis

Abstract

Chronic myelogenous leukemia (CML) is a clonal disorder characterized by proliferation of cells that possess the bcr-abl fusion gene resulting in the production of one of two possible chimeric 210-kDa tyrosine kinase proteins. Since these chimeric proteins are expressed only in leukemic cells they have the potential to serve as tumor-specific antigens for cytotoxic T lymphocytes (CTL). Using the 12B1 murine leukemia cell line, derived by retroviral transformation of BALB/c bone marrow cells with the bcr-abl (b3a2) fusion gene, we have demonstrated that intravenous inoculation of 12B1 cells into BALB/c mice results in a disseminated acute leukemia analogous to human CML in blast crisis. Histological sections of liver and spleen and polymerase chain reaction analysis of peripheral blood, bone marrow, liver, spleen and lymph nodes confirmed the presence of bcr-abl+ leukemia cells in these murine tissues, while Western blot data demonstrated the expression of the fusion protein in 12B1 cells. Immunization of mice with dendritic cells (DC) loaded with the synthetic bcr-abl chimeric nonapeptide, GFKQSSKAL, led to a 150 times higher frequency of bcr-abl-specific CTL precursors in the spleen than in mice immunized with peptide alone. I...Continue Reading

Citations

May 12, 2005·Cancer Immunology, Immunotherapy : CII·Yi ZengEmmanuel Katsanis
Apr 14, 2006·Cancer Immunology, Immunotherapy : CII·Nicolas LarmonierEmmanuel Katsanis
May 1, 2007·Current Hematologic Malignancy Reports·Richard E Clark
Jan 23, 2003·Cancer Biotherapy & Radiopharmaceuticals·Xueshu ZhangJim Xiang
Feb 6, 2014·Journal of Immunotoxicology·Jacek Rolinski, Iwona Hus
Jun 4, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Michael W GranerEmmanuel Katsanis
Mar 20, 2015·Biological & Pharmaceutical Bulletin·Hai-Bo XiYu Li
Mar 25, 2008·Chemico-biological Interactions·Dhanasekaran SugapriyaPanchanatham Sachdanandam
Jul 11, 2013·BioMed Research International·Yanan QinYangqiu Li
Jul 15, 2015·BioMed Research International·Takenori UeharaHeiichiro Udono
Mar 1, 2006·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Xinchun ChenEmmanuel Katsanis
Jun 25, 2016·Chinese Journal of Integrative Medicine·Shanthi PalaniveluSachdanandam Tiruvaiyaru Panchanadham
Apr 30, 2014·Bone Marrow Transplantation·Y ZengE Katsanis
Sep 22, 2006·International Journal of Cancer. Journal International Du Cancer·Yi ZengEmmanuel Katsanis
Mar 1, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kerri L KislinEmmanuel Katsanis
Apr 8, 2004·International Journal of Cancer. Journal International Du Cancer·Yi ZengEmmanuel Katsanis
Nov 3, 2004·Cancer Research·Michael W GranerEmmanuel Katsanis

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