Dendritic cells from C57BL/6 mice undergo activation and induce Th1-effector cell responses against Campylobacter jejuni.

Microbes and Infection
Vijay A K RathinamLinda S Mansfield

Abstract

Food-borne Campylobacter jejuni (Cj) is an important cause of enteritis. We showed that C57BL/6 and congenic interleukin (IL)-10(-/-) mice serve as models of Cj colonization and enteritis, respectively. Thus, C57BL/6 mice are resistant to Cj induced disease. Because dendritic cells (DCs) are central to regulating adaptive immune responses, we investigated the interaction of Cj with murine bone marrow-derived DCs (BM-DCs) to assess bacterial killing, DC activation, and the ability of Cj-infected BM-DCs to stimulate Campylobacter-specific T cell responses in vitro. BM-DCs challenged with Cj efficiently internalized and killed Cj 11168 and significantly upregulated surface MHC-II, CD40, CD80 and CD86 demonstrating a mature phenotype. Infected BM-DCs secreted significant amounts of tumor necrosis factor-alpha (TNF-alpha), IL-6 and IL-12p70. Formalin-killed Cj also induced maturation of BM-DCs with similar cytokine production but at a significantly lower magnitude than live bacteria. Maximal activation of murine BM-DCs required internalization of Cj; attachment alone was not sufficient to elicit significant responses. Also, various strains of Cj elicited different magnitudes of cytokine production from BM-DCs. Finally, in a cocultur...Continue Reading

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Citations

Aug 25, 2011·Medical Microbiology and Immunology·Anna RoseMargaret J Dallman
Mar 31, 2010·Nature Immunology·Vijay A K RathinamKatherine A Fitzgerald
Jan 21, 2010·Infection and Immunity·David R TribbleA Louis Bourgeois
Oct 23, 2013·Cell Communication and Signaling : CCS·Derrick R SamuelsonMichael E Konkel
Jun 4, 2015·Virulence·Anja KlančnikDarinka Vučković
Oct 11, 2015·Microbial Pathogenesis·Alexia Papadopoulos, Jean-Pierre Gorvel
May 27, 2014·International Immunopharmacology·Jong-Jin KimSung-Tae Yee
Apr 19, 2018·Animal Health Research Reviews·Phillip T Brooks, Linda S Mansfield
Jul 27, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sandhya GanesanKatherine A Fitzgerald
Oct 1, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lieneke I BouwmanJos P M van Putten
Nov 9, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ravichandran PanchanathanDivaker Choubey

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