Dendritic cells matured in the presence of the lycopodium alkaloid annotine direct T cell responses toward a Th2/Treg phenotype

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Ingibjorg HardardottirJona Freysdottir

Abstract

Annotine is a lycopodane-type alkaloid isolated from the Icelandic club moss Lycopodium annotinum ssp. alpestre. Annotine does not inhibit acetylcholinesterase, as some other lycopodium alkaloids do, and other bioactivities have not been reported. The aim of this study was to determine the effects of annotine on maturation of dendritic cells (DCs) and their ability to activate allogeneic CD4(+) T cells. Human monocyte-derived DCs were matured in the absence or presence of annotine at a concentration of 1, 10 or 100 μg/ml. The effect of the annotine on maturation of the DCs was determined by measuring concentration of cytokines in culture supernatant by ELISA and expression of surface molecules by flow cytometry. DCs matured in the absence or presence of annotine at 100 µg/ml were also co-cultured with allogeneic CD4(+) T cells and concentration of cytokines in supernatants determined by ELISA and expression of surface molecules by flow cytometry. When cultured alone, DCs matured in the presence of annotine secreted less of the pro-inflammatory cytokines IL-6 and IL-23 and had a tendency toward less secretion of IL-12p40 than DCs matured in the absence of annotine. However, when DCs were matured in the presence of annotine and t...Continue Reading

References

Mar 25, 1998·Current Biology : CB·G J McKenzieA N McKenzie
Jul 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Luciano AdoriniMilan Uskokovic
Nov 27, 2004·Natural Product Reports·Xiaoqiang Ma, David R Gang
Apr 9, 2005·European Journal of Immunology·Philippe GossetFrançois Trottein
Feb 24, 2006·Infection and Immunity·Ulrike Meltzer, David Goldblatt
Sep 12, 2006·Journal of Ethnopharmacology·Ilkay OrhanErdem Yesilada
Sep 19, 2006·International Immunopharmacology·Sesselja OmarsdottirJona Freysdottir
Jun 21, 2008·Chemico-biological Interactions·Hai Yan ZhangXi Can Tang
Apr 21, 2009·Medicinal Research Reviews·Raju Gautam, Sanjay M Jachak
Feb 16, 2010·Nature Reviews. Immunology·Margarida Saraiva, Anne O'Garra
Feb 10, 2012·Journal of Natural Products·David J Newman, Gordon M Cragg
Nov 19, 2013·The Journal of Pharmacy and Pharmacology·Eduardo Luis KonrathAmélia T Henriques
Jan 31, 2014·Frontiers in Microbiology·Brodie MilesChristopher W Cutler
Aug 26, 2014·Nature Reviews. Immunology·Sarah L GaffenDaniel J Cua

❮ Previous
Next ❯

Citations

May 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dongke MaXin Di
Aug 4, 2019·Neurochemistry International·Leila MohtashamiBehjat Javadi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.