Dendritic cells pulsed with exogenous hepatitis B surface antigen particles efficiently present epitopes to MHC class I-restricted cytotoxic T cells

European Journal of Immunology
Detlef StoberR Schirmbeck

Abstract

L(d)- and K(b)-binding epitopes processed by murine dendritic cells (DC) pulsed with exogenous, particulate hepatitis B surface antigen (HBsAg) are presented to cytotoxic T lymphocytes (CTL). The specific and dose-dependent induction of IFN-gamma release and cytotoxicity in CTL by metabolically active DC did not depend on antigenic peptides contaminating the particles, was cytochalasin D resistant, independent of the maturation state of DC, and blocked by primaquine, amiloride and NH(4)Cl (indicating involvement of acid proteolysis). The specific immunostimulatory phenotype of pulsed DC was maintained for about 3 h after the end of the pulse but rapidly decayed thereafter. Processing of L(d)- and K(b)-binding epitopes from exogenous HBsAg particles by pulsed DC for presentation was TAP independent. Surface-associated 'empty' (presentation-deficient) 64(+) L(d) molecules (defined by the mAb 64-3-7), but not trimeric (presentation-competent) 30(+) L(d) molecules (defined by the mAb 30-5-7) had to be available during the pulse of DC with exogenous HBsAg particles to generate 30(+) L(d)molecules that present the antigenic S(28-39) peptide. Exogenous beta2-microglobulin present during the pulse of DC with HBsAg particles facilitated...Continue Reading

References

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Citations

Dec 4, 2003·Journal of Hepatology·Nektarios DikopoulosJörg Reimann
Apr 23, 2003·Microbes and Infection·Georg LautschamNeil Blake
Aug 3, 2013·The Journal of Clinical Investigation·Adam J GehringAntonio Bertoletti
Jun 29, 2010·Nature Immunology·Lionel Le BourhisOlivier Lantz
Aug 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Víctor Gabriel MorónClaude Leclerc
Feb 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christian WahlJörg Reimann
Nov 5, 2014·Cellular & Molecular Immunology·Adam J Gehring, June Ann D'Angelo
May 4, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nektarios DikopoulosJörg Reimann
Nov 1, 2015·Archivum Immunologiae Et Therapiae Experimentalis·Katerina ZachovaMilan Raska
Jan 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eunyoung ChunKi-Young Lee
Feb 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Detlef StoberJörg Reimann
Aug 12, 2003·Expert Review of Vaccines·Florence BoisgéraultClaude Leclerc
Mar 10, 2004·Expert Opinion on Biological Therapy·Masoud H ManjiliJohn R Subjeck

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