Dendritic cells transfected with hepatocellular carcinoma (HCC) total RNA induce specific immune responses against HCC in vitro and in vivo

Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
B H XieQiang He

Abstract

Immunotherapy is an effective method for preventing metastasis and recurrence of carcinoma. Hepatocellular carcinoma (HCC) is a common malignancy with a high rate of recurrence, and has not successfully been introduced to immunotherapy. Peripheral blood mononuclear cells were isolated from whole blood of HCC patients and stimulated to transform into dendritic cells (DCs). These DCs were then transfected with RNA extracted from HepG-2 hepatoma cells to induce expression of specific antigens. The transfected DCs stimulated T lymphocytes to produce cytotoxic T lymphocytes, which specifically attacked HepG-2 cells. Injection of T lymphocytes from HCC patients and transfected DCs into severe combined immunodeficiency mice limited the growth of HepG-2 tumors. A specific immune response against hepatoma can be generated in vivo by administering DCs transfected with RNA from a specific tumor. This method may have therapeutic application in humans to reduce recurrence of HCC.

References

Aug 1, 1996·The Journal of Experimental Medicine·D BoczkowskiE Gilboa
Apr 29, 1998·Biochimica Et Biophysica Acta·K KarikóD J Langer
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A HeiserJ Vieweg
Jun 3, 2000·Annual Review of Immunology·J BanchereauK Palucka
Sep 21, 2000·Immunology Letters·M Nouri-ShiraziK Palucka
Nov 9, 2000·The Journal of Clinical Investigation·D A Mitchell, S K Nair
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S KoidoJ Gong
Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A HeiserJ Vieweg
May 31, 2001·Experimental Dermatology·J Saenz-BadillosR D Granstein
Jul 11, 2001·Immunologic Research·J E Ohm, D P Carbone
Aug 28, 2001·Journal of Hematotherapy & Stem Cell Research·L GarderetM Lopez

❮ Previous
Next ❯

Citations

Jul 3, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Oleg V MarkovMarina A Zenkova
Aug 26, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yasunari Nakamoto
May 10, 2018·World Journal of Gastroenterology : WJG·Naofumi Mukaida, Yasunari Nakamoto
Jun 9, 2017·Molecular and Cellular Biochemistry·Vikrant RaiDevendra K Agrawal

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Related Papers

Ryōikibetsu shōkōgun shirīzu
M Ebara
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
Di-Peng OuZhi-Gang Chang
© 2021 Meta ULC. All rights reserved