Dendritic-like streptavidin/alkaline phosphatase nanoarchitectures for amplified electrochemical sensing of DNA sequences

Langmuir : the ACS Journal of Surfaces and Colloids
Fausto LucarelliMarco Mascini

Abstract

This study describes the development and characterization of a novel dendritic-like signal amplification pathway. Such an analytical strategy relies on the use of streptavidin and biotinylated alkaline phosphatase, which can be simply and conveniently self-assembled to build nanoarchitectures rich in enzyme labels. The performance of this enzyme-based amplification route was demonstrated in connection with the electrochemical sensing of DNA sequences. Compared to the commercially available streptavidin-conjugated alkaline phosphatase labels, a single generation of the streptavidin/biotinylated alkaline phosphatase assembly allowed a 15-20-fold enhancement of the electroanalytical signals. The higher sensitivity allowed by the dendritic-like route was attributed to the lower steric hindrance of the proteins employed for this amplification path. As low as 50 pmol/L of a 388-bp-long amplicon identifying Salmonella spp. was easily detected. The experimental results additionally demonstrated that the sensitivity of the method could be further increased in a linear fashion with the number of protein-enzyme generations.

References

Mar 20, 1991·Journal of Molecular Biology·E E Kim, H W Wyckoff
Dec 9, 1998·Analytical Chemistry·A B SteelM J Tarlov
Jan 29, 2002·Analytical Chemistry·Hanbin MaoPaul S Cremer
Nov 18, 2003·Analytical Chemistry·Joseph WangQiyu Zhu
May 29, 2004·Analytical Chemistry·Elena DomínguezArántzazu Narváez
Aug 17, 2004·Biosensors & Bioelectronics·Guido CarpiniMarco Mascini
Oct 1, 2005·Analytical Chemistry·Maria Lisa Del GialloMarco Mascini

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Citations

Feb 23, 2013·Analytical and Bioanalytical Chemistry·Anne WalterGerd-Uwe Flechsig
Mar 19, 2013·Journal of Virological Methods·Xiuzhen ShengWenbin Zhan
Jun 3, 2008·Analytical Biochemistry·Muhammad J A ShiddikyYoon-Bo Shim
Feb 12, 2008·Analytica Chimica Acta·Fausto LucarelliMarco Mascini
Nov 26, 2013·Journal of Colloid and Interface Science·Biebele Abel, Kadir Aslan
Jan 22, 2009·Lab on a Chip·Kazuo Hosokawa, Mizuo Maeda
Jun 27, 2018·Sensors·Giulia SelvoliniGiovanna Marrazza
Jan 19, 2013·Chemical Communications : Chem Comm·Yu W ChuJames R Carey
Nov 10, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kazuo Hosokawa
May 21, 2008·Journal of the American Chemical Society·Benoît LimogesBernd Schöllhorn
May 5, 2007·Analytical Chemistry·Rebeca Miranda-CastroPaulino Tuñón-Blanco
Jul 7, 2007·Analytical Chemistry·Kazuo HosokawaMizuo Maeda

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