Dendritic spines and pre-synaptic boutons are stable despite local deep hypothermic challenge and re-warming in vivo.

PloS One
Yicheng XieTimothy H Murphy

Abstract

Deep hypothermia to 20°C is used clinically for major pediatric and adult surgical procedures. In particular, it is used in the "standstill operation" where blood flow is stopped for up to 30 min. Patients recovering from these procedures can exhibit neurological deficits. Such deficits could arise from changes to dendritic spines and plasticity-induced changes in network function as a result of cooling and/or re-warming. In the brain, each dendritic spine represents a single excitatory synapse and their number can be reflective of injury or plasticity-induced changes in network function. This research sought to determine whether deep hypothermia and re-warming have detrimental effects on synaptic stability and network function. In vivo 2-photon (2-P) imaging in green/yellow fluorescent protein (GFP/YFP)-expressing transgenic mice was performed to determine whether 4 hours of deep hypothermia and 2 hours of re-warming can have relatively covert effects on dendritic spine and presynaptic bouton stability. At the same time, electroencephalographic (EEG) activity was recorded to evaluate network function during deep hypothermia and re-warming. We report that deep hypothermia and subsequent re-warming did not change the stability o...Continue Reading

References

Sep 1, 1979·Stroke; a Journal of Cerebral Circulation·P A SteenJ D Michenfelder
Sep 1, 1991·Surgical Neurology·C J BakerR A Solomon
Nov 1, 1990·The Annals of Thoracic Surgery·S DeLeonR Sulayman
Dec 1, 1996·The Journal of Thoracic and Cardiovascular Surgery·R M EngelmanP S Pekow
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·D KleinfeldW Denk
Dec 14, 1999·The Journal of Thoracic and Cardiovascular Surgery·C D KurthR Raghupathi
Dec 20, 2002·Nature·Jaime GrutzendlerWen-Biao Gan
Jun 3, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shengxiang ZhangTimothy H Murphy
Mar 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ania K MajewskaMriganka Sur
May 4, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Darren L Clark, Frederick Colbourne
May 26, 2006·Cerebral Cortex·R David AndrewSergei A Kirov
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Liping Liu, Midori A Yenari
Apr 13, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Craig E BrownTimothy H Murphy
Apr 27, 2007·Neurosurgery·William J MackRobert A Solomon
Feb 15, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Timothy H MurphyRichard Liu
Feb 17, 2009·Journal of Neurotrauma·Crystal L MacLellanFrederick Colbourne
Oct 22, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ricardo MostanyCarlos Portera-Cailliau
Oct 23, 2010·Nature Methods·Patrick J DrewDavid Kleinfeld
Mar 4, 2011·Recent Patents on Inflammation & Allergy Drug Discovery·Jong-Heon KimKyoungho Suk
Dec 15, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sherri TranTimothy H Murphy
Feb 2, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Andy Y ShihDavid Kleinfeld

❮ Previous
Next ❯

Citations

Dec 15, 2020·Biological Reviews of the Cambridge Philosophical Society·Zhe ShiXin-Min Liu

❮ Previous
Next ❯

Software Mentioned

Clampfit
AxoScope
Matlab2010

Related Concepts

Related Feeds

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.