Dendrobium Officinale Kimura et Migo Ameliorates Insulin Resistance in Rats with Diabetic Nephropathy

Medical Science Monitor Basic Research
Ming Zhao, Jungang Han

Abstract

BACKGROUND Emerging evidence suggests the potential of Dendrobium officinale Kimura et Migo (DO) in treating the complications of diabetes mellitus (DM). We evaluated the therapeutic potential of DO in treating diabetic nephropathy (DN) by preventing insulin resistance. MATERIAL AND METHODS A DN model was established. Mean glomerular volume of rats was estimated by the method of Weibel-Gomez. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to determine the expression of mRNAs and we used Western blot assay to determine the expression of proteins. The levels of fasting insulin (FINS) and glucagon (GLU) were measured and we assessed the levels of high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-a (TNF-a), and interleukin-6 (IL-6) using the enzyme-linked immunosorbent assay (ELISA). RESULTS Compared with the Normal rats, the levels of urinary glucose, albuminuria, Scr, albuminuria/Scr and BUN, and the expression levels of CaN, TLR-2, TLR-4, MyD88, hs-CRP, TNF-a, and IL-6, the level of FINS, GLU, and HOMAIR were increased in DN, DO 1.0, DO 2.0, and DMBG groups. Compared with the DN rats, in DO 1.0, DO 2.0, and DMBG groups the glomerular volume was smaller, the levels of urinary glu...Continue Reading

Citations

Mar 7, 2020·Journal of Zhejiang University. Science. B·Zhi-Feng ZhouJin-Lei Lv
Jun 15, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Shan-Shan LeiSu-Hong Chen
Aug 8, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jie SunYan Yang
Jan 3, 2020·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·Miaoyuan ZhangLin Zhou
Jan 28, 2022·Natural Products and Bioprospecting·Ding-Kang ChenJiang-Miao Hu

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Methods Mentioned

BETA
light microscopy
PCR
electrophoresis

Software Mentioned

SPSS

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