Dengue virus infection induces formation of G3BP1 granules in human lung epithelial cells

Archives of Virology
Jun XiaPing Zhang

Abstract

Cells reprogram ongoing translation in response to viral infection, resulting in formation of stress granules (SGs), while viruses have evolved a variety of strategies to antagonize the host SG response. Previous literature reported that in BHK-1 cells, infection with dengue virus (DENV) interfered with the SG formation. In the current study, we further investigated SG formation in human epithelial A549 cells by detecting subcellular localization of two SG hallmarks, TIA-1 and G3BP1. In response to DENV type 2 (DENV2) and type 3 (DENV3) infection, G3BP1, but not TIA-1, was recruited into cytoplasmic granules in some cells, and viral protein synthesis was significantly impaired in the G3BP1-granule-containing cells. Knockdown of G3BP1 significantly rescued the dsRNA-mediated suppression of DENV2 replication. Furthermore, our data showed that the phosphorylation of protein kinase regulated by dsRNA (PKR) and eIF2α, as well as accumulation of dsRNA, mainly occurred at the late stage of viral infection. This work revealed that in DENV-infected A549 cells, G3BP1 granules were assembled independently of TIA-1 and had a negative impact on viral replication. This extends our understanding of the antagonistic relationship between the SG...Continue Reading

References

Dec 1, 1992·The Journal of General Virology·E Hatada, R Fukuda
Jul 17, 1998·Clinical Microbiology Reviews·D J Gubler
Sep 1, 2000·Microbes and Infection·W J McBride, H Bielefeldt-Ohmann
Oct 17, 2002·Cell Stress & Chaperones·Paul Anderson, Nancy Kedersha
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Scot R KimballHeather P Harding
Mar 8, 2006·The Journal of Cell Biology·Paul Anderson, Nancy Kedersha
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mohamed M Emara, Margo A Brinton
Nov 13, 2007·Lancet·Scott B Halstead
Feb 23, 2008·Trends in Biochemical Sciences·Paul Anderson, Nancy Kedersha
Apr 15, 2008·Cell Host & Microbe·Carla J Beckham, Roy Parker
Oct 7, 2008·Nature Cell Biology·Kyoko ArimotoMutsuhiro Takekawa
Mar 10, 2011·The Journal of Biological Chemistry·José Peña, Eva Harris
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Takbum Ohn, Paul Anderson
Dec 29, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Denys A KhaperskyyCraig McCormick
Mar 13, 2012·Trends in Microbiology·James P White, Richard E Lloyd
Apr 13, 2012·The New England Journal of Medicine·Cameron P SimmonsBridget Wills
May 17, 2012·Viruses·Juliet MorrisonAna Fernandez-Sesma
Apr 5, 2013·Wiley Interdisciplinary Reviews. RNA·Richard E Lloyd
Jul 16, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chia-Ling ChenYee-Shin Lin
Dec 10, 2013·Journal of Molecular Biology·Angela M GreenEva Harris

❮ Previous
Next ❯

Citations

Jul 2, 2016·Viruses·Natalia Poblete-DuránFernando Valiente-Echeverría
Jan 10, 2018·Frontiers in Plant Science·Kristiina MäkinenMaija Pollari
Nov 9, 2018·Journal of Virology·Linda J VisserFrank J M van Kuppeveld
May 1, 2019·Virologica Sinica·Qiang ZhangMingzhou Chen
Apr 30, 2019·Wiley Interdisciplinary Reviews. RNA·Shaun T CrossJeffrey Wilusz
Jul 4, 2017·Nature Reviews. Immunology·Craig McCormick, Denys A Khaperskyy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.