Denitrifier community composition along a nitrate and salinity gradient in a coastal aquifer

Applied and Environmental Microbiology
Alyson E SantoroChristopher A Francis

Abstract

Nitrogen flux into the coastal environment via submarine groundwater discharge may be modulated by microbial processes such as denitrification, but the spatial scales at which microbial communities act and vary are not well understood. In this study, we examined the denitrifying community within the beach aquifer at Huntington Beach, California, where high-nitrate groundwater is a persistent feature. Nitrite reductase-encoding gene fragments (nirK and nirS), responsible for the key step in the denitrification pathway, were PCR amplified, cloned, and sequenced from DNAs extracted from aquifer sediments collected along a cross-shore transect, where groundwater ranged in salinity from 8 to 34 practical salinity units and in nitrate concentration from 0.5 to 330 muM. We found taxonomically rich and novel communities, with all nirK clones exhibiting <85% identity and nirS clones exhibiting <92% identity at the amino acid level to those of cultivated denitrifiers and other environmental clones in the database. Unique communities were found at each site, despite being located within 40 m of each other, suggesting that the spatial scale at which denitrifier diversity and community composition vary is small. Statistical analyses of nir ...Continue Reading

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Citations

Jul 25, 2009·Microbial Ecology·Jennifer W EdmondsMary Ann Moran
Apr 9, 2011·World Journal of Microbiology & Biotechnology·Monnat TheerachatWarawut Chulalaksananukul
Apr 30, 2013·Environmental Science and Pollution Research International·Maria BarrettVincent O'Flaherty
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