Dense Layer of Bacteriophages Ordered in Alternating Electric Field and Immobilized by Surface Chemical Modification as Sensing Element for Bacteria Detection

ACS Applied Materials & Interfaces
Łukasz RichterRobert Hołyst

Abstract

Faster and more sensitive environmental monitoring should be developed to face the worldwide problem of bacterial infections. To remedy this issue, we demonstrate a bacteria-sensing element that utilizes dense and ordered layers of bacteriophages specific to the given bacteria strain. We combine (1) the chemical modification of a surface to increase the surface coverage of bacteriophages (2) with an alternating electric field to greatly increase the number of properly oriented bacteriophages at the surface. Usually, in sensing elements, a random orientation of bacteriophages results in steric hindrance, which results in no more than a few percent of all receptors being available. An increased number of properly ordered phages results in the optimal performance of phage receptors, manifesting in up to a 64-fold increase in sensitivity and a limit of detection as low as 100 CFU mL-1. Our sensing elements can be applied for selective, sensitive, and fast (15 min) bacterial detection. A well-studied pair T4 bacteriophage-bacteria Escherichia coli, was used as a model; however, the method could be adapted to prepare bacteriophage-based sensors for detection of a variety of bacterial strains.

References

Aug 1, 1977·Biopolymers·J G De La Torre, V A Bloomfield
Dec 1, 1992·European Journal of Biochemistry·P SchwindI Rasched
Mar 1, 1994·Virology·N K AbuladzeF A Eiserling
Mar 17, 1999·Journal of Molecular Recognition : JMR·L OlofssonI A Nicholls
Oct 20, 2006·Archives of Virology·H-W Ackermann
Dec 2, 2009·Applied and Environmental Microbiology·M TolbaM W Griffiths
Feb 11, 2014·Expert Review of Medical Devices·Irina SorokulovaVitaly Vodyanoy
Mar 22, 2014·ACS Applied Materials & Interfaces·Jin-Hee HanIan M Kennedy
Mar 25, 2014·The Analyst·R G van der MerweN C Gey van Pittius
Jul 7, 2014·Colloids and Surfaces. B, Biointerfaces·Zeinab HosseinidoustNathalie Tufenkji
Oct 17, 2015·Analytical and Bioanalytical Chemistry·Riikka PeltomaaMaría Cruz Moreno-Bondi
Dec 20, 2016·Bioconjugate Chemistry·Marta JanczukRobert Hołyst
Jan 27, 2017·Annual Review of Food Science and Technology·H AnanyM W Griffiths

❮ Previous
Next ❯

Citations

Sep 22, 2019·Nanomaterials·James J TronoloneSaniya LeBlanc
Jul 22, 2018·Scientific Reports·Ewa BrzozowskaJoanna Niedziółka-Jönsson
Dec 11, 2019·Micromachines·Jingting XuYi-Kuen Lee
Oct 3, 2020·Nanomaterials·Jan Paczesny, Krzysztof Bielec
Jan 12, 2021·Biosensors & Bioelectronics·Wajid HussainShenqi Wang
Mar 5, 2021·Bioconjugate Chemistry·Caitlin M CarmodySam R Nugen
May 1, 2021·Pharmaceuticals·Daniel Rosner, Jason Clark
May 27, 2021·ACS Biomaterials Science & Engineering·Larry O'ConnellYoann Roupioz
May 15, 2018·ACS Sensors·Mandana AmiriSabine Szunerits

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.