Dental follicle mesenchymal stem cells down-regulate Th2-mediated immune response in asthmatic patients mononuclear cells

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
Deniz GencTunc Akkoc

Abstract

Asthma is a chronic inflammatory disease in which inflammatory responses have the polarisation of CD4+ T cells to Th2 cells. Dental follicle mesenchymal stem cells (DFSCs) have strong anti-inflammatory properties comparable to other mesenchymal stem cells. We investigated the immunomodulatory effects of DFSCs on CD4+ T helper cell responses of asthmatic patients and compared the results with those obtained with asthmatic subjects on immunotherapy and with healthy individuals. Peripheral blood mononuclear cells (PBMC) were isolated from immunotherapy naïve asthmatics, asthmatics on subcutaneous Der p1 immunotherapy and from healthy individuals. PBMC were pre-conditioned with anti-CD3/anti-CD28 mAbs, Der p1 or IFN-γ in the presence and absence of DFSCs and analysed for T cell viability and proliferation, CD4+ CD25+ FOXP3+ regulatory T cell frequencies, cytokine expression, and GATA3, T bet and FoxP3 expressions. Neutralisation of TGF-β and blockade of IDO and PGE2 pathways were performed to determine suppressive signalling pathways of DFSCs. Dental follicle mesenchymal stem cells suppressed proliferative responses of CD4+ T lymphocytes and increased the frequency of Treg cells. DFSCs decreased effector and effector memory CD4+ T ...Continue Reading

References

Sep 30, 2009·International Archives of Allergy and Immunology·Vincent LombardiOmid Akbari
Feb 17, 2010·Immunology and Cell Biology·William E Paul
Jun 4, 2011·The Journal of Allergy and Clinical Immunology·Saleh Al-MuhsenQutayba Hamid
Jun 7, 2011·The Journal of Allergy and Clinical Immunology·Sevgi KelesIsil Barlan
Jul 30, 2011·The Journal of Biological Chemistry·Anil B Mukherjee, Zhongjian Zhang
Aug 25, 2011·Stem Cell Research & Therapy·Michelle M DuffyMatthew D Griffin
Jul 28, 2012·Dental Clinics of North America·Reuben H KimMo K Kang
Apr 2, 2013·The Journal of Allergy and Clinical Immunology·Clare M Lloyd, Sejal Saglani
Jan 29, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ananda S MirchandaniFoo Y Liew
Jul 6, 2014·Allergy, Asthma & Immunology Research·Chae-Seo Rhee
Sep 27, 2014·Respirology : Official Journal of the Asian Pacific Society of Respirology·John G ElliotAlan L James
Oct 27, 2014·Respiratory Research·Yukari Asai-TajiriYoichi Nakanishi
Nov 27, 2014·World Journal of Stem Cells·Dobroslav KyurkchievStanimir Kyurkchiev
Dec 3, 2014·The Journal of Allergy and Clinical Immunology·Moisés A CalderónPascal Demoly
Mar 17, 2015·The Journal of Allergy and Clinical Immunology·Emma S ChambersCatherine M Hawrylowicz
Mar 20, 2015·Immunology and Cell Biology·Lauren CharronAnne L Astier
May 6, 2015·Molecular Medicine Reports·Shao-Lin ZengJiong Yang
May 30, 2015·The World Allergy Organization Journal·Cezmi A Akdis, Mübeccel Akdis
Dec 19, 2015·The European Respiratory Journal·Antoine FroidureCharles Pilette
Aug 9, 2016·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Larry Borish
Jun 2, 2017·Frontiers in Bioengineering and Biotechnology·Cristina SobacchiCiro Menale

❮ Previous
Next ❯

Citations

Apr 11, 2019·Stem Cells and Development·Jie ZhangChunmiao Jiang
Jun 4, 2020·Immunotherapy·Tunç Akkoç, Deniz Genç
Oct 23, 2019·Stem Cells International·Tao ZhouXin Zhou
Aug 26, 2019·Allergology International : Official Journal of the Japanese Society of Allergology·He LiWenru Su

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.