Dental Pulp Cells Isolated from Teeth with Superficial Caries Retain an Inflammatory Phenotype and Display an Enhanced Matrix Mineralization Potential

Frontiers in Physiology
Hanaa AlkharobiReem El-Gendy

Abstract

We have isolated dental pulp cells (DPCs) from three healthy (hDPCs) and three carious (cDPCs) donors and shown that compared to hDPCs cells isolated from superficial carious lesions show higher clonogenic potential; show an equivalent proportion of cells with putative stem cell surface markers; show enhanced matrix mineralization capability; have enhanced angiogenic marker expression and retain the inflammatory phenotype in vitro characteristic of superficial caries lesions in vivo. Our findings suggest that cDPCs may be used for further investigation of the cross talk between inflammatory, angiogenic and mineralization pathways in repair of carious pulp. In addition cells derived from carious pulps (almost always discarded) may have potential for future applications in mineralized tissue repair and regeneration.

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Citations

Apr 28, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Qiaoqiao JinZhengwei Huang
May 1, 2018·Frontiers in Bioengineering and Biotechnology·Hanaa Esa AlkharobiReem El-Gendy
Jun 11, 2019·Tissue Engineering. Part B, Reviews·Karim M Fawzy El-SayedChristof E Dörfer
Sep 19, 2021·Tissue Engineering and Regenerative Medicine·Hua-Nien LeeWei-Dong Tian

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Methods Mentioned

BETA
FACS
flow cytometry

Software Mentioned

FACS DivA
Prism

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