Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y

International Endodontic Journal
S A KilloughC R Irwin

Abstract

To investigate whether dental pulp fibroblasts express neuropeptide Y (NPY) and NPY-Y1 in vitro and to determine the effects of the cytokines including interleukin-1β (IL-1β), TGF- β(1) , substance P and NPY on the expression of NPY Y1. Three primary fibroblast cell strains were obtained from freshly extracted human third molar teeth. RT-PCR was utilized to detect expression of NPY and mRNA expression. Membrane protein samples were isolated, and protein expression was determined by Western blotting. Radioimmunoassay was used to quantify NPY expression in healthy (n = 35) and carious (n = 39) whole pulp samples, and the student's t-test was used to test for statistical significance. In addition, the 3-(4,5-Dimethylthiazol,2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to assay fibroblast cell growth. mRNA transcripts were found in all three fibroblast cell populations with the cytokines having a stimulatory effect on its expression (P < 0.05). NPY mRNA was not detected in the cell strains. NPY-Y1 receptor protein expression was visualized by Western blotting, and there was no effect of IL-1β or TGF- β(1) on its expression. The mean concentration of NPY-Ir determined by radioimmunoassay in non-carious teeth was 19.40 ...Continue Reading

References

Jul 29, 1998·Archives of Oral Biology·R UddmanL Edvinsson
Apr 24, 2003·Journal of Dental Research·K M HargreavesD L Jackson
Jun 24, 2003·Drug News & Perspectives·Stefan Boehm
Apr 3, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·F.T. Lundy, G.J. Linden
Sep 15, 2004·Trends in Immunology·Sammy BedouiTakashi Yamamura
Apr 25, 2006·European Journal of Oral Sciences·Ikhlas A El KarimFionnuala T Lundy
May 26, 2006·Journal of Dental Research·S R Haug, K J Heyeraas
Dec 23, 2006·Dental Clinics of North America·Michael A Henry, Kenneth M Hargreaves
Feb 27, 2007·Journal of Endodontics·Chin-Lo Hahn, Frederick R Liewehr
Aug 14, 2008·International Endodontic Journal·I A El KarimF T Lundy
Nov 18, 2008·Archives of Oral Biology·Fionnuala T LundyGerard J Linden
Dec 17, 2008·Journal of Endodontics·Simon A KilloughChris R Irwin

❮ Previous
Next ❯

Citations

Jan 12, 2013·Current Protein & Peptide Science·Daria AugustyniakFionnuala T Lundy
Sep 21, 2020·Journal of Endodontics·Fionnuala T LundyIkhlas A El Karim
May 1, 2021·International Journal of Molecular Sciences·Daria AugustyniakFionnuala T Lundy
Nov 14, 2021·Journal of Periodontal Research·Lewis WinningFionnuala T Lundy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.