Dental Pulp Stem Cell-Derived Factors Alleviate Subarachnoid Hemorrhage-Induced Neuroinflammation and Ischemic Neurological Deficits

International Journal of Molecular Sciences
Te-Fu ChenChia-Yang Shiau

Abstract

Aneurysmal subarachnoid hemorrhage (aSAH), characterized by the extravasation of blood into the subarachnoid space caused by an intracranial aneurysm rupture, may lead to neurocognitive impairments and permanent disability and usually carries poor outcome. Dental or gingiva-derived stem cells have been shown to contribute to immune modulation and neuroregeneration, but the underlying mechanisms are unclear. In the present study, we sought to investigate whether dental pulp stem cells (DPSCs) secrete certain factor(s) that can ameliorate the neural damage and other manifestations in a rat aSAH model. Twenty-four hours after the induction of aSAH, microthrombosis, cortical vasoconstriction, and the decrease in microcirculation and tissue oxygen pressure were detected. Intrathecal administration of DPSC-derived conditioned media (DPSC-CM) ameliorated aSAH-induced vasoconstriction, neuroinflammation, and improved the oxygenation in the injured brain. Rotarod test revealed that the aSAH-induced cognitive and motor impairments were significantly improved by this DPSC-CM administration. Cytokine array indicated the major constituent of DPSC-CM was predominantly insulin growth factor-1 (IGF-1). Immunohistochemistry staining of injured ...Continue Reading

References

Jul 1, 1994·Stroke; a Journal of Cerebral Circulation·J P BroderickA Leach
Jan 2, 1997·The New England Journal of Medicine·W I Schievink
Jun 19, 1998·Brain Research. Molecular Brain Research·D ItoS Kohsaka
Jan 27, 2006·The New England Journal of Medicine·Jose I SuarezWarren R Selman
Nov 26, 2009·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Eileen Maloney-Wilensky, Peter Le Roux
Apr 27, 2012·Acta Cirúrgica Brasileira·Lee Tae-Hoon, Lee Yoon-Seok
Jun 12, 2012·Endocrinology and Metabolism Clinics of North America·Ignacio Torres Aleman
Oct 27, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bo Hua HuDonald Coling
Mar 30, 2013·Stroke; a Journal of Cerebral Circulation·Cindy T J van VelthovenDonna M Ferriero
Aug 12, 2014·BioMed Research International·Brandon A MillerGustavo Pradilla
Nov 2, 2014·Journal of Neuroinflammation·Agustina NoaillesNicolás Cuenca
Mar 5, 2016·Frontiers in Neuroscience·Vanesa Nieto-EstévezCarlos Vicario-Abejón
Oct 27, 2016·Brain and Behavior·Yogi Chang-Yo HsuanMao-Tsun Lin
Nov 16, 2016·Current Stem Cell Research & Therapy·Katari Venkatesh, Dwaipayan Sen
Feb 17, 2017·Frontiers in Cellular Neuroscience·Sarah WrigleyDaniela Tropea
May 10, 2017·Balsaeng'gwa saengsig·Hee Jung Kim, Jeong-Soo Park
Aug 26, 2017·International Journal of Molecular Sciences·Francisco J VizosoRoman Perez-Fernandez
May 18, 2018·Frontiers in Neurology·Airton Leonardo de Oliveira Manoel, R Loch Macdonald
Jun 2, 2018·Stem Cells International·Lihua LuoQingsong Ye
Sep 8, 2018·Scientific Reports·Kuo-Chuan WangSung-Tsang Hsieh
Sep 27, 2018·Stem Cells International·Firdos Alam KhanSarah Ameen Almofty

❮ Previous
Next ❯

Methods Mentioned

BETA
dissection
ELISA
Flow Filtration
Protein Assay
membrane

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.