Dental pulp tissue engineering with bFGF-incorporated silk fibroin scaffolds

Journal of Biomaterials Applications
Jing-Wen YangZhi Chen

Abstract

The clinical translation of regenerative endodontics demands further development of suitable scaffolds. Here, we assessed the possibility of using silk fibroin scaffold for pulp regeneration with dental pulp stem cells (DPSCs) and basic fibroblast growth factor (bFGF) in ectopic root canal transplantation model. Porous silk fibroin scaffolds were fabricated using freeze-drying technique (with or without bFGF incorporation), and characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy. DPSCs were isolated, characterized, seeded onto scaffolds, and inserted into the tooth root fragments. Cell viability and morphology were tested in the 3D model in vitro using CCK8 assay and SEM. Furthermore, the ectopic transplantation model was used to verify the generation of pulp-like tissue in DPSCs seeded silk fibroin scaffold with bFGF, as examined by histological analysis. DPSCs seeded in silk fibroin scaffold survived, exhibited cytoplasmic elongation in scaffolds at least 4 weeks in culture. bFGF promoted DPSCs viability in tooth fragments/scaffolds (TSS) between 7 and 28 days. Pulp-like tissue was generated in the bFGF-incorporated TSS with DPSCs. Histologically, the generated tissue was shown to b...Continue Reading

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Citations

Mar 26, 2016·Frontiers in Physiology·Jingwen YangZhi Chen
Aug 6, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Yu-Chih ChiangChun-Pin Lin
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Methods Mentioned

BETA
scanning electron microscopy
dissection

Software Mentioned

SPSS

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