Dentin matrix components extracted with phosphoric acid enhance cell proliferation and mineralization

Dental Materials : Official Publication of the Academy of Dental Materials
Satin SalehiJack L Ferracane

Abstract

Acids, such as those used in adhesive dentistry, have been shown to solubilize bioactive molecules from dentin. These dentin matrix components (DMC) may promote cell proliferation and differentiation, and ultimately contribute to dentin regeneration. The objective of this study was to evaluate the potential for varying concentrations of DMC extracted from human dentin by phosphoric acid of a range of pHs to stimulate proliferation and mineralization of two different cultured pulp cell populations. DMC were solubilized from powdered human dentin (7 days - 4°C) by phosphoric acid of pH 1, 3, and 5 and also, EDTA. Extracts were dialyzed for 7 days against distilled water and lyophilized. Undifferentiated mouse dental pulp cells (OD-21) and cells of the odontoblast-like cell line (MDPC-23) were seeded in six-well plates (1×10(5)) and cultured for 24h in DMEM (Dulbecco's modified Eagle's medium) containing 10% (v/v) FBS (fetal bovine serum). The cells were washed with serum-free medium and then treated with different concentrations of DMC (0.01, 0.1, 1.0 and 10.0μg/ml) daily in serum free medium for 7 days. After 3, 5 (MDPC-23 only), and 7 days of treatment, cell proliferation was measured using 10vol% Alamar blue solution, which wa...Continue Reading

References

Nov 1, 1992·Matrix : Collagen and Related Research·W T ButlerR A Foster
Sep 1, 1991·Calcified Tissue International·A L Boskey, B L Dick
Apr 16, 1998·European Journal of Oral Sciences·W T Butler
Aug 6, 1998·Journal of Immunological Methods·M M NociariC Russo
Nov 5, 1999·Archives of Biochemistry and Biophysics·H G GargR J Linhardt
Feb 10, 2000·Journal of Dentistry·D TziafasH Lesot
Oct 18, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·G EmberyM Goldberg
Jul 31, 2002·Journal of Dental Research·S GronthosS Shi
Feb 6, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Michel Goldberg, Anthony J. Smith
Jun 10, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C QinW T Butler
Feb 22, 2012·The Journal of Biological Chemistry·Satoru HayanoTakashi Yamashiro
Oct 30, 2012·Journal of Dental Research·A Tezvergil-MutluayD H Pashley

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Citations

Dec 18, 2016·Journal of Endodontics·Manuel Marí-BeffaAránzazu Díaz-Cuenca
Dec 5, 2019·Journal of Applied Oral Science : Revista FOB·Anabela B PaulaEunice Carrilho
May 27, 2021·Journal of Dental Research·N S RodriguesL E Bertassoni
Aug 28, 2021·International Journal of Molecular Sciences·Zhuo XieZhengmei Lin

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