Dentin sialophosphoprotein-derived proteins in porcine pulp and dentin - Gene expression and function

Journal of Oral Biosciences
Ryuji Yamamoto, Yasuo Yamakoshi

Abstract

Dentin sialophosphoprotein (DSPP) is the most abundant non-collagenous protein in dentin and is critical for the proper mineralization of tooth dentin. DSPP is processed by proteases into three major domains: dentin sialoprotein (DSP), dentin glycoprotein (DGP) and dentin phosphoprotein (DPP). Two mRNA variants are expressed from the Dspp gene. The larger transcript encodes full-length DSPP (DSP+DGP+DPP). The shorter transcript encodes only DSP. We fractionated DSPP-derived proteins from the dental pulp of developing porcine incisors using heparin chromatography. DSP was identified, but little DPP could be detected in any fraction. Expression of full-length Dspp mRNA, determined by qPCR analysis, was significantly higher in odontoblasts than in pulp. Expression of DSP-only mRNA was almost equal in odontoblasts and in the body of pulp. Expression of full-length Dspp mRNA was also significantly higher than expression of DSP-only mRNA in odontoblasts. Both the full-length and DSP-only Dspp mRNA showed only trace expression in the pulp tip. We purified TGF-β1-unbound or -bound to DPP and DSP using high performance liquid chromatography (HPLC) and measured its alkaline phosphatase stimulating activity in human periodontal cells with...Continue Reading

Citations

Aug 20, 2021·Science and Technology of Advanced Materials·Jun-Ichi SasakiSatoshi Imazato

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