Dentinal proteoglycans demonstrate an increasing order of affinity for hydroxyapatite crystals during the transition of predentine to dentine

Calcified Tissue International
Anna M MilanR J Waddington

Abstract

The transition from an unmineralized predentine to a mineralized dentine involves a variety of molecular extracellular matrix interactions and protein degradation events. Previous studies have identified that different pools of proteoglycan (PG) species are present within the matrix of the predentine, the transitional phase at the predentine-dentine border, and the mineralized dentine. These PGs alter with respect to the chemical nature of the glycosaminoglycan (GAG) chain and as a result of extracellular processing of the macromolecule in the matrix. This study has examined the hydroxyapatite (HAP) binding affinity of the PGs isolated from these phases and the influence of the attached GAG chains upon their binding characteristics. PGs isolated from the three phases were characterized to contain a mixture of decorin and biglycan, substituted with chondroitin sulfate GAG chain(s). Maximal binding for dentine PGs onto HAP was achieved at 15.60 microg/ml protein and for predentine-dentine interface PGs at 0.125 mg/ml. A significantly increasing gradient of affinity was observed moving toward dentine, with dentine PGs exhibiting 19 times greater binding affinity for HAP than predentine PGs and 7.5 times greater affinity than prede...Continue Reading

References

Mar 1, 1979·Journal of Dental Research·D I Hay, E C Moreno
May 1, 1992·Bone and Mineral·A L BoskeyZ Schwartz
Jan 1, 1989·Modern Problems of Pharmacopsychiatry·B Casu
Feb 1, 1987·Calcified Tissue International·W G Stetler-Stevenson, A Veis
Jan 1, 1984·Calcified Tissue International·E C MorenoD I Hay
Mar 15, 1984·The Biochemical Journal·F RahemtullaW T Butler
Jun 1, 1995·Biomaterials·D T WassellG Embery
May 1, 1993·Calcified Tissue International·R J WaddingtonR C Hall
Apr 1, 1996·Archives of Oral Biology·N YoshibaH Ozawa
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·R V Iozzo
Oct 18, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·G EmberyM Goldberg
Mar 22, 2003·European Journal of Oral Sciences·Ulrika PeterssonMikael Wendel
Jun 5, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·R J WaddingtonD M Lloyd

❮ Previous
Next ❯

Citations

Feb 9, 2010·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Heiko RichterHorst Kierdorf
Jun 17, 2005·Archives of Oral Biology·Tsuneyoshi SanoMasanori Nakamura
Sep 26, 2006·Micron : the International Research and Review Journal for Microscopy·Paula DechichiAntônio Wilson de Ameida
Sep 12, 2009·The Open Dentistry Journal·Khansa Taha Ababneh, Taiseer Hussain Al-Khateeb
Nov 19, 2004·Calcified Tissue International·Anna M MilanRachel J Waddington
May 29, 2020·Proceedings of the National Academy of Sciences of the United States of America·Magdalena WojtasEli D Sone

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.