Dentinogenesis and Tooth-Alveolar Bone Complex Defects in BMP9/GDF2 Knockout Mice

Stem Cells and Development
Xia HuangHongmei Zhang

Abstract

Tooth development is regulated by sequential and reciprocal epithelium-mesenchymal interactions and their related molecular signaling pathways, such as bone morphogenetic proteins (BMPs). Among the 14 types of BMPs, BMP9 (also known as growth differentiation factor 2) is one of the most potent BMPs to induce osteogenic differentiation of mesenchymal stem cells. The purpose of this study was to examine potential roles of BMP9 signaling in tooth development. First, we detected the expression pattern of BMP9 in tooth germ during postnatal tooth development, and we found that BMP9 was widely expressed in odontoblasts, ameloblasts, dental pulp cells, and osteoblasts in alveolar bones. Then, we established a BMP9-KO mouse model. Gross morphological examination revealed that the tooth cusps of BMP9-KO mice were significantly abraded with shorter roots. Micro-computed tomography and three-dimensional reconstruction analysis indicated that the first molars of the BMP9-KO mice exhibited a reduced thickness dentin, enlarged pulp canals, and shortened roots, resembling the phenotypes of the common hereditary dental disease dentinogenesis imperfecta. Further, the alveolar bone of the BMP9-KO mutants was found to be shorter and had a decreas...Continue Reading

References

Feb 25, 2003·Journal of Dental Research·T YamashiroI Thesleff
Feb 4, 2005·American Journal of Medical Genetics. Part a·Juan DongMary MacDougall
Feb 10, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hue H LuuTong-Chuan He
Sep 18, 2008·The Journal of Medical Investigation : JMI·Keiko MiyoshiTakafumi Noma
Apr 18, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Dobrawa NapieralaBrendan Lee
Sep 1, 2012·Journal of Periodontal Research·M P GibsonC Qin
Oct 12, 2012·Journal of Periodontal Research·C H BaeE S Cho
Dec 20, 2012·Development·Shihai JiaRulang Jiang
Dec 22, 2012·International Journal of Oral Science·Afsaneh RangianiJian-Quan Feng
Feb 1, 2013·Journal of Tissue Engineering and Regenerative Medicine·Xue ZhangFu-Zhai Cui
Feb 1, 2013·Journal of Tissue Engineering and Regenerative Medicine·Sijia NaYan Jin
Feb 22, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Antonius L J J BronckersBernd Schröder
Jun 29, 2013·International Journal of Oral Science·Audrey RakianStephen E Harris
Oct 12, 2013·European Journal of Oral Sciences·Monica P GibsonChunlin Qin
Nov 14, 2013·Stem Cells and Development·Young-Man LeeEun-Cheol Kim
Mar 13, 2014·Current Cancer Drug Targets·Ruidong LiZhong-Liang Deng
Jul 17, 2014·Stem Cells and Development·Shiliang GuoHelena H Ritchie
Aug 15, 2014·European Journal of Human Genetics : EJHG·Muriel de La Dure-MollaAriane Berdal
Jun 11, 2015·BioMed Research International·Bryan D Johnston, Wendy E Ward
Oct 28, 2015·Journal of Dental Research·Y ChenA George
Mar 5, 2016·Journal of Dental Research·L W ZhangL L Wu
Apr 22, 2016·Journal of Molecular Histology·Hye-Kyung LeeJoo-Cheol Park
May 4, 2017·Journal of International Society of Preventive & Community Dentistry·Antoine CassiaRobert Cavézian

❮ Previous
Next ❯

Citations

Jan 9, 2020·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yan-Man ZhouHong-Yan Zhao
Jun 9, 2020·Biochemical Society Transactions·Liu LiuDingming Huang

❮ Previous
Next ❯

Methods Mentioned

BETA
Transgenic
genotyping
biopsies
Protein Assay
PCR

Software Mentioned

SPSS
Image Processing Language
micro
Mimics
CT

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.