Dentipain, a Streptococcus pyogenes IdeS protease homolog, is a novel virulence factor of Treponema denticola.

Biological Chemistry
Kazuyuki IshiharaJan Potempa

Abstract

Treponema denticola is a major pathogen of chronic periodontitis. Analysis of the T. denticola genome revealed a gene orthologous with a cysteine protease-encoding gene from Streptococcus pyogenes (IdeS). IdeS interferes with IgG-dependent opsonophagocytosis by specific cleavage of IgG molecules. Analysis of this gene (termed ideT) revealed it to encode a two-domain protein whose N-terminus is composed of tandem immunoglobulin-like domains followed by a C-terminal IdeS-like protease domain. In this study we show that during secretion the IdeT protein is processed into an N-terminal fragment which remains associated with the cell, and a C-terminal part released into the medium. Although the secreted domain of IdeT, termed dentipain, shows only 25% identity to the IdeS protease, the putative catalytic cysteine and histidine residues are strongly conserved. Recombinant dentipain cleaves the insulin β-chain, an activity which is inhibited by E-64, a diagnostic inhibitor of cysteine proteases. Apart from insulin no cleavage of other protein substrates was detected, suggesting that dentipain has oligopeptidase activity. A mutant strain was constructed expressing a modified IdeT variant, the dentipain domain of which was deleted. This...Continue Reading

References

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Citations

Mar 1, 2012·Journal of Oral Microbiology·J Christopher Fenno
Mar 19, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M B Visser, R P Ellen
May 18, 2017·The Journal of Biological Chemistry·Theodoros GoulasF Xavier Gomis-Rüth
Jan 18, 2012·Journal of Innate Immunity·Ulrich von Pawel-Rammingen
Oct 20, 2018·The Bulletin of Tokyo Dental College·Yuichiro KikuchiKazuyuki Ishihara
Jun 27, 2017·NPJ Biofilms and Microbiomes·Jyoti Chhibber-GoelAmit Sharma

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