Deoxycorticosterone biosynthesis from progesterone in kidney tissue of the human fetus

The Journal of Clinical Endocrinology and Metabolism
C A WinkelP C MacDonald

Abstract

The levels of deoxycorticosterone (DOC) and DOC sulfate are extraordinarily high in the umbilical cord plasma of human newborns. However, the sources of DOC and DOC sulfate in the human fetus are not defined. Recently, it was shown that plasma progesterone was converted to DOC in extraadrenal tissues in pregnant, nonpregnant, and adrenalectomized women and in men. Thus, DOC formation from plasma progesterone constitutes another example of the formation of a biologically active steroid hormone from a circulating precursor. In addition, the conversion of [3H]progesterone to [3H]DOC in homogenates and microsome-enriched preparations of adult human kidney tissue was demonstrated. To investigate the origin of DOC in the human fetus, we sought 1) to ascertain whether steroid 21-hydroxylase activity was present in human fetal kidney tissue and 2) to establish the kinetics of the reaction catalyzed by this enzyme should it be present in fetal kidney. We employed microsome-enriched preparations of kidney tissue obtained from human abortuses of 9-20 weeks gestation. [3H]DOC formation from [3H]progesterone (1 microM) proceeded in a linear fashion for 3 h. and the rate of formation of [3H]DOC from [3H]progesterone (1 microM) was linear wit...Continue Reading

Citations

Sep 15, 1982·Klinische Wochenschrift·K HierholzerI Witt
Aug 1, 1983·Journal of Steroid Biochemistry·A GueramiP C MacDonald
Oct 1, 1983·Journal of Steroid Biochemistry·M L Casey, P C MacDonald
Jul 1, 1988·Journal of Steroid Biochemistry·K NahoulR Scholler
Oct 1, 1989·Journal of Steroid Biochemistry·K NahoulL Dehennin
Nov 1, 1989·Journal of Steroid Biochemistry·G T GriffingJ C Melby
Dec 16, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Vincenzo PezziBruce R Carr
Sep 1, 2001·The Journal of Steroid Biochemistry and Molecular Biology·D RogoffA Slominski
Aug 1, 1982·The Journal of Clinical Investigation·M L Casey, P C MacDonald
Jan 1, 1982·Clinical and Experimental Hypertension. Part A, Theory and Practice·I AntonipillaiR Horton
Sep 1, 1986·Clinical Endocrinology·S LocheM I New
Jul 1, 1986·The Journal of Urology·D B KarpfG D Braunstein

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