PMID: 8962060Dec 10, 1996Paper

Deoxycytidine kinase and deoxyguanosine kinase of Lactobacillus acidophilus R-26 are colinear products of a single gene

Proceedings of the National Academy of Sciences of the United States of America
N MaD H Ives

Abstract

Three of the four deoxynucleoside kinases required for growth of Lactobacillus acidophilus R-26 exist as heterodimeric pairs specific for deoxyadenosine (dAK) and deoxycytidine (dCK) or dAK and deoxyguanosine (dGK). However, only two tandem genes, dak/dgk, are found, and are expressed only as dAK/dGK in transformed Escherichia coli. Sequencing peptides spanning 63% of the native dCK subunit revealed a sequence identical to that deduced from dgk (beginning MTVIVL...), except that dCK lacks residues 2 and 3 (dCK is M..IVL; dGK is .TVIVL). Also, mass spectrometry indicates that native dCK and dGK subunits are identical in mass adjusted for the first three residues. Furthermore, the native enzymes have identical isoelectric pH values, indicating an equal number of charged residues. To enable E. coli to express peptide having the native dCK sequence, codons 2 and 3 were deleted from the dgk portion of the tandem genes, resulting in expression of protein having the specificities and regulatory properties of native dAK/dCK, including heterotropic stimulation of dAK activity by deoxycytidine or dCTP (not deoxyguanosine or dGTP) and end-product inhibition of the respective activities by dATP and dCTP. Subcloning normal and mutant dgk yi...Continue Reading

References

May 29, 1992·Cell·A Varshavsky
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L W Hardy, E Nalivaika
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·E G ChottinerB S Mitchell
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P H HirelS Blanquet
Jan 1, 1995·Pharmacology & Therapeutics·E S Arnér, S Eriksson

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