Deoxyhypusine synthase gene is essential for cell viability in the yeast Saccharomyces cerevisiae

FEBS Letters
K SasakiM Miyazaki

Abstract

Deoxyhypusine synthase catalyzes the first of two steps in the biosynthesis of hypusine, a modification of a specific lysine residue in the precursor of eukaryotic translation initiation factor 5A. We have purified deoxyhypusine synthase from yeast, and cloned and sequenced the corresponding gene encoding a 387-amino acid protein from Saccharomyces cerevisiae. Gene disruption experiments indicated that the deoxyhypusine synthase gene is essential for cell growth in yeast. This gene was shown to be an intron-free, single-copy gene, and its product can catalyze the synthesis of deoxyhypusine equally in two precursor forms of eIF-5A, derived from two distinct genes of yeast.

References

Apr 15, 1979·Journal of Molecular Biology·R Piñon
Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Nov 1, 1995·Molecular and Cellular Biology·Z Guo, F Sherman
Apr 14, 1995·The Journal of Biological Chemistry·E C WolffM H Park

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Citations

Jul 13, 2002·International Journal of Cancer. Journal International Du Cancer·Paul M J ClementMyung Hee Park
Jun 24, 2008·Molecular Genetics and Genomics : MGG·Mariana C FrigieriSandro R Valentini
Dec 6, 2011·Amino Acids·M CaragliaA Abbruzzese
Aug 24, 2010·Nature Structural & Molecular Biology·Tatsuo YanagisawaShigeyuki Yokoyama
Nov 19, 2003·European Journal of Biochemistry·Paul M J ClementHans E Johansson
Mar 26, 2013·The Journal of Biological Chemistry·Suong NguyenMargaret A Phillips
Aug 16, 2000·The EMBO Journal·G LipowskyD Görlich
Oct 18, 2006·BMC Microbiology·James T NjugunaAnnette E Kaiser
Dec 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jong-Hwan ParkMyung Hee Park
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yunfei HuangSolomon H Snyder
Nov 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Manas K ChattopadhyayHerbert Tabor
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·D Ober, T Hartmann
Aug 15, 2013·Amino Acids·Imke von Koschitzky, Annette Kaiser
Sep 30, 2015·Molecular Microbiology·Jürgen LassakKirsten Jung
Mar 17, 2010·Plant Physiology and Biochemistry : PPB·Christine FuellAnthony J Michael
Jan 23, 2009·Journal of Cellular Physiology·Alexander GosslauKuang Yu Chen
Jun 1, 2011·The FEBS Journal·Eva GreganovaPeter Bütikofer
Mar 8, 2006·The FEBS Journal·Paul M J ClementMyung H Park
Jul 18, 2014·Critical Reviews in Biochemistry and Molecular Biology·Thomas E DeverByung-Sik Shin
Jul 15, 2015·Journal of Molecular Biology·Leonor Miller-FlemingMarkus Ralser
Jun 4, 2014·Molecular and Biochemical Parasitology·Nimisha MittalRentala Madhubala
Jul 29, 2004·Molecular and Biochemical Parasitology·Ilka M MolitorGabriele M König
Jun 24, 2017·Nucleic Acids Research·Byung-Sik ShinThomas E Dever

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