Deoxynivalenol inhibits the expression by goblet cells of intestinal mucins through a PKR and MAP kinase dependent repression of the resistin-like molecule β

Molecular Nutrition & Food Research
Philippe PintonMarc Maresca

Abstract

The food-associated mycotoxin deoxynivalenol (DON) is known to affect intestinal functions. However, its effect on intestinal mucus is poorly characterized. We analyzed the effects of DON on human goblet cells (HT29-16E cells) and porcine intestinal explants. Results showed that subtoxic doses of DON (as low as 1 μM) decreased mucin (MUC) production. qPCR analysis demonstrated that this inhibition was due to a specific decrease in the level of mRNA encoding for the intestinal membrane-associated (MUC1) and the secreted MUCs (MUC2, MUC3). Mechanistic studies demonstrated that DON effect relied on the activation of the protein kinase R and the mitogen-activated protein kinase p38 ultimately leading to the inhibition of the expression of resistin-like molecule beta, a known positive regulator of MUC expression. Taken together, our results show that at low doses found in food and feed, DON is able to affect the expression and production of MUCs by human and animal goblet cells. Due to the important role of MUCs in the barrier function and in the interaction of commensal bacteria with the host, such effect could explain the observed modifications in the microbial diversity and the increased susceptibility to enteric infection follow...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 8, 2005·Veterinary Immunology and Immunopathology·Sandrine Bouhet, Isabelle P Oswald
Feb 27, 2008·Inflammatory Bowel Diseases·Rim Belharbi KrimiJean-Claude Marie
Mar 3, 2009·American Journal of Respiratory Cell and Molecular Biology·Daniel J AngeliniRoger A Johns
Apr 1, 2009·International Journal of Molecular Sciences·Yann J WachéPhilippe Fravalo
Jul 18, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Martine Kolf-ClauwIsabelle P Oswald
May 15, 2010·Toxicon : Official Journal of the International Society on Toxinology·Marc Maresca, Jacques Fantini
Aug 13, 2010·Current Gastroenterology Reports·Young S Kim, Samuel B Ho
Jul 13, 2011·Glia·Helisoa RazafimanjatoMarc Maresca
Aug 11, 2011·The European Respiratory Journal·C FangS Ying
Nov 18, 2011·Trends in Microbiology·Nathalie Juge
Sep 14, 2012·Journal of Clinical Gastroenterology·Franco ScaldaferriAntonio Gasbarrini
Oct 11, 2012·Molecular Nutrition & Food Research·Franz BerthillerJoerg Stroka
Dec 22, 2012·Beneficial Microbes·P Brandtzaeg
Jan 1, 2013·Toxicology Letters·Chanemougasoundharam Arunachalam, Fiona M Doohan
Mar 13, 2013·Nature Reviews. Gastroenterology & Hepatology·Malin E V JohanssonGunnar C Hansson
Jun 12, 2013·Glycobiology·Kirk S B Bergstrom, Lijun Xia
Jun 19, 2013·Critical Reviews in Food Science and Nutrition·Stoycho D Stoev
Jan 16, 2014·Annual Review of Food Science and Technology·Felicia WuJames J Pestka
May 27, 2014·Toxins·Philippe Pinton, Isabelle P Oswald
Sep 23, 2014·Molecular Nutrition & Food Research·Johannes GerdingHans-Ulrich Humpf
Oct 30, 2014·Journal of Applied Toxicology : JAT·Khaled GhareebQendrim Zebeli

❮ Previous
Next ❯

Citations

Dec 19, 2015·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Bogdan LewczukMaciej Gajęcki
Dec 4, 2015·Journal of Agricultural and Food Chemistry·Gunther AntonissenSiska Croubels
Feb 13, 2016·Journal of Applied Microbiology·F GrazianiJ Perrier
Jun 25, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Nathan BroekaertSiska Croubels
Jul 16, 2016·Archives of Toxicology·Peyman AkbariJohanna Fink-Gremmels
Sep 25, 2016·Archives of Toxicology·Delphine PayrosIsabelle P Oswald
May 4, 2017·Journal of Applied Toxicology : JAT·Zhao PengWei Yang
Jan 16, 2018·Molecular Nutrition & Food Research·Chengcheng RenPaul de Vos
Jan 23, 2019·Molecular Nutrition & Food Research·Xiaojun LiJinyou Duan
Jun 9, 2019·International Journal of Molecular Sciences·Zhihua RenJunliang Deng
Jun 22, 2017·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Hervé RobertIsabelle P Oswald
Nov 21, 2017·Archives of Toxicology·Qinghua WuKamil Kuca
Sep 27, 2019·Journal of Biochemical and Molecular Toxicology·Zhigang ChenHongyu Lei
Oct 2, 2020·Toxins·Yanan GaoNan Zheng
Oct 11, 2017·Archives of Toxicology·Gisela Romina GarcíaIsabelle P Oswald
Mar 16, 2019·Toxins·Imourana Alassane-KpembiIsabelle P Oswald
Sep 11, 2017·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Lutz Edler
Mar 21, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ana Paula F L BracarenseIsabelle P Oswald
May 1, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Shuai WangDesheng Qi
Aug 15, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·You ZhouZhouping Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical Society Transactions
B J Van KlinkenJ Dekker
Toxicological Sciences : an Official Journal of the Society of Toxicology
Fabien GrazianiMarc Maresca
© 2022 Meta ULC. All rights reserved