Deoxyribonucleic acid flow cytometry in the assessment of spermatogenesis

The Journal of Urology
A KostakopoulosN Tamvakis

Abstract

We compared deoxyribonucleic acid (DNA) flow cytometric analysis of testicular tissue to quantitative assessment of spermatogenesis. We studied 35 infertile men with azoospermia or oligospermia. All patients underwent incisional testicular biopsies. DNA flow cytometric analysis was performed on each specimen to evaluate the ability of the method to quantify alterations in spermatogenesis. The results were compared to quantitative histological examination. At least 100 spermatic tubules were examined on each specimen and the number of spermatids per tubule was counted. All histological specimens were examined by the same pathologist. Of the 35 specimens analyzed with DNA flow cytometry 5 were normal, while the percentage of haploid cells (spermatids and spermatozoa) was decreased (hypospermatogenesis) in 14, complete maturation arrest was noted in 2 and almost complete absence of haploid cells was found in 14. Comparing the findings on histological examination with histograms, excellent correlation was noted in cases of the Sertoli-cell-only syndrome and complete maturation arrest, while 3 of 14 histograms with hypospermatogenesis demonstrated normal spermatogenesis on histological examination. Additionally 1 of 5 histograms wit...Continue Reading

Citations

Dec 10, 1999·The American Journal of Surgical Pathology·M NistalR Paniagua
Aug 13, 2005·Biological Reviews of the Cambridge Philosophical Society·C Marc LuetjensJoachim Wistuba
Dec 6, 2012·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Brian P EnrightGuenter Blaich
Jan 1, 1997·International Reviews of Immunology·I A Heijnen, J G van de Winkel

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