PMID: 7371644Apr 1, 1980Paper

Deoxyribonucleic acid methyltransferase from the eukaryote, Chlamydomonas reinhardi

European Journal of Biochemistry
H Sano, R Sager

Abstract

DNA methyltransferase was purified 310-fold from a green alga, Chlamydomonas reinhardi vegetative cells. The native enzyme of molecular weight 55 000--58 000 catalyzed the transfer of methyl groups from S-adenosylmethionine to the 5 position of cytosine in DNA. Native DNA accepted methyl groups 10-fold more than did denatured DNA. The sequence specificity analysis of methylated deoxycytidine in vitro revealed that the enzyme introduces methyl groups preferentially into sequences containing 5'd(T-mC-R)3'. Kinetic analysis of the reaction indicated that the enzyme obeys a random sequential mechanism. The extent of saturation with methyl groups depends upon the species from which the DNA was obtained. Kinetic analysis of the reaction catalyzed by RNA polymerase II has indicated that DNA methylation decreases the rate of initiation of RNA synthesis, but does not affect the rate of RNA chain elongation.

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Citations

May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H SanoR Sager
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M GraessmannD Simon
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T Y Feng, K S Chiang
Aug 17, 1987·European Journal of Biochemistry·G TheissH Follmann
Mar 8, 2006·Urologic Oncology·Zhengwang ZhangJer-Tsong Hsieh
Jan 1, 1986·Differentiation; Research in Biological Diversity·P MaharajanE Scarano
Sep 15, 1983·European Journal of Biochemistry·H SanoR Sager
Jan 1, 1982·CRC Critical Reviews in Biochemistry·R L Adams, R H Burdon
Jan 30, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R L AdamsH M Yesufu
Aug 2, 1983·Biochimica Et Biophysica Acta·G P PfeiferD Drahovsky
Jun 14, 1991·Biochemical and Biophysical Research Communications·M GiordanoG Pedrali-Noy
Sep 30, 1982·Biochemical and Biophysical Research Communications·E S LyonW B Jakoby
Jun 19, 1981·Science·M Ehrlich, R Y Wang

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