Deoxyribonucleic acid-protein interactions associated with transcriptional initiation of the mouse testis-specific cytochrome c gene

Biology of Reproduction
G K YiuNorman B Hecht

Abstract

Transcriptional regulation of the mouse testis-specific cytochrome c (cyt. cT) gene was studied by examining DNA-protein interactions in its proximal promoter. Testicular and liver nuclear proteins bound to the cyt. cT gene at sites -105 to -81, +87 to +113, and +146 to +169, suggesting interactions with ubiquitous nuclear proteins. Protein present in liver nuclear extracts bound to a fourth site at -176 to -125, whereas protein present in testicular nuclear extracts bound to a subregion of this site at -176 to -140. The sequence from -136 to -127, bound by liver but not testicular nuclear proteins, is similar to that of the binding site of a somatic c-mos repressor protein. Lastly, different nuclear proteins from mouse liver and testis bound to a region from -18 to +31 that contains a putative Y box at -13 to -2. Mobility shift assays, Southwestern blots, and immunoprecipitation studies have established that this putative Y box binds a 52-kDa mouse testicular homologue of the Xenopus germ cell-specific Y-box protein and a competing 50-kDa protein present in both liver and testis nuclear extracts. These data suggest that the testis-specific expression of the mouse cyt. cT gene during spermatogenesis may be regulated by the diff...Continue Reading

Citations

Nov 19, 1997·Molecular Reproduction and Development·M R Sairam, V S Subbarayan
Mar 26, 1999·Molecular Reproduction and Development·M Ambhaikar, E Goldberg
May 21, 2008·Molecular and Cellular Biology·Wei GuRobert H Singer
Mar 15, 2008·Fertility and Sterility·Sue HammoudDouglas T Carrell
Apr 17, 1999·The Journal of Biological Chemistry·J H Oosterhuis, F A van der Hoorn
Sep 22, 2001·Biology of Reproduction·J YuR M Schultz

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