Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Xiaona LiuDefang Li

Abstract

Deoxyschizandrin (DS) is a bioactive benzocyclooctadiene lignan found in the fruit of Schisandra chinensis. However, poor bioavailability and non-specificity of DS frequently caused low therapeutic efficacy. In the present study, DS-liposome (DS-lipo) was implemented to enhance the hepatic targeting and inhibition effects on adipocyte differentiation in 3T3-L1 cells. The formulations enabled encapsulation of as much as 24.14% DS. The DS-lipo prepared was about 73.08 nm, as measured by laser light scattering (LLS) morphology. In the visual field of a scanning electron microscope (SEM), the liposomes were spherical with similar size and uniform dispersion. Fluorescence live imaging study exhibited hepatic targeting of liposomes in vivo. Furthermore, High-Content Analysis (HCS) imaging microassay analyses revealed DS-lipo and DS reduced cytoplasmic lipid droplet in 3T3-L1 adipocytes, with the IC50 value of 8.68 μM and 31.08 μM, respectively. The lipid droplet accumulation inhibition rate of 10 μM DS-lipo was above 90%, which was even superior to the effect of 30 μM DS solution. The current findings suggest that DS-lipo was a therapeutic strategy for alleviating lipid-associated diseases and nonalcoholic fatty liver disease (NAFLD).

References

Mar 1, 1985·The Journal of Cell Biology·P GreenspanS D Fowler
Jan 22, 2000·Annals of Internal Medicine·S BellentaniC Tiribelli
Oct 20, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Noriyuki MaedaNaoto Oku
Aug 19, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·John M LukSheung-Tat Fan
Oct 25, 2007·Clinical Pharmacology and Therapeutics·L ZhangO C Farokhzad
Jun 3, 2008·Journal of Ethnopharmacology·Alexander Panossian, Georg Wikman
Jun 28, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Hyunah ChoGlen S Kwon
Aug 5, 2011·Accounts of Chemical Research·Wafa' T Al-Jamal, Kostas Kostarelos
Mar 20, 2012·Analytical Biochemistry·Katja ZebischMatthias Brandsch
Jan 29, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gitanjali SharmaJagdish Singh
May 24, 2013·Journal of Liposome Research·Cintia Maria Saia CeredaEneida de Paula
Aug 21, 2013·Expert Opinion on Therapeutic Patents·José L Arias
Sep 18, 2013·Nature Reviews. Gastroenterology & Hepatology·Rohit Loomba, Arun J Sanyal
Jan 28, 2014·Biomaterials·Jia YuXianfeng Chen
Dec 4, 2014·World Journal of Gastroenterology : WJG·Nur Arslan
Dec 24, 2014·New Biotechnology·Erik Reimhult
Feb 7, 2015·Current Pharmaceutical Biotechnology·Biswajit MukherjeeChowdhury M Hossain
Feb 14, 2015·International Journal of Nanomedicine·Giuseppina Bozzuto, Agnese Molinari
Mar 23, 2015·International Journal of Pharmaceutics·Rikhav P GalaMohamed A Alhnan
Jun 16, 2016·Journal of Biomedical Nanotechnology·Lei JiangZhongwei Gu
Jun 21, 2016·Biochemical and Biophysical Research Communications·Shifeng WangYanjiang Qiao

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence
fluorescence imaging
Light
dynamic light scattering
light scattering

Software Mentioned

GraphPad Prism
Bruker MISE

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Hepatology
Constance E Ruhl, James E Everhart
Revista de gastroenterología de México
Raúl Bernal Reyes
The Journal of the Association of Physicians of India
Rakesh K Tandon
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Rui-dan ZhengMin-de Zeng
© 2022 Meta ULC. All rights reserved