PMID: 4612537Dec 1, 1974

Deoxysubstitution in RNA by RNA polymerase in vitro: a new approach to nucleotide sequence determinations

Proceedings of the National Academy of Sciences of the United States of America
G V PaddockW Salser

Abstract

Deoxynucleotides have been incorporated into RNA synthesized in vitro by RNA polymerase with either double-stranded or single-stranded DNA as a template. By use of this technique to block or promote cleavage at a particular phosphodiester bond, a variety of specific cleavages may be obtained with the available ribonucleases and deoxyribonuclease I. These methods should greatly increase the ease and rapidity of nucleotide sequence determinations.

References

Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R PoonF Ramirez
Feb 14, 1967·Journal of Molecular Biology·G G Brownlee, F Sanger
Feb 9, 1972·Nature: New Biology·I M VermaD Baltimore
Feb 9, 1972·Nature: New Biology·A BankP A Marks
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·K FryF T Hatch
Aug 7, 1972·Biochemical and Biophysical Research Communications·J HurwitzS Wickner
Jan 1, 1974·Annual Review of Biochemistry·W Salser
Dec 1, 1969·European Journal of Biochemistry·G G Brownlee, F Sanger
Sep 1, 1965·Journal of Molecular Biology·F SangerB G Barrell
Nov 12, 1970·Biochimica Et Biophysica Acta·F T Hatch, J A Mazrimas
Feb 1, 1966·Analytical Biochemistry·S B Zimmerman, D Sandeen
Aug 1, 1974·Nucleic Acids Research·A Van de VoordeW Fiers

Citations

Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A Blank, C A Dekker
Aug 1, 1981·Environmental Health Perspectives·K W Jennette
Feb 1, 1975·CRC Critical Reviews in Biochemistry·R WuE Jay

Related Concepts

Autoradiography
Alkaline DNase
Deoxyribonucleotides
DNA, Double-Stranded
DNA, Single-Stranded
Electrophoresis
Alkalescens-Dispar Group
Oligonucleotides
Pancreas
Phosphorus Radioisotopes

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