Dependence of 13C NMR chemical shifts on conformations of rna nucleosides and nucleotides

Journal of Magnetic Resonance
M EbrahimiG S Harbison

Abstract

Cross-polarization magic-angle spinning solid-state NMR spectroscopy has been used to investigate the dependence of (13)C sugar chemical shifts on specific conformational parameters of a variety of ribonucleotides and ribonucleosides. Solid-state NMR is a valuable tool for nucleoside and nucleotide structural studies since it provides the means to acquire spectra that correspond to single conformations, as opposed to (13)C solution NMR methods. The distinct effects of sugar puckering on the C1', C4', and C5' resonances of C2' endo (S type) and C3' endo (N type) furanoid conformations allow us to separate them into two groups. Further analysis of each group reveals an additional dependence of the C1' and C5' resonances on the glycosidic and C4'-C5' exocyclic torsion angles, respectively. However, it is found that the glycosidic conformation cannot independently be determined from sugar (13)C chemical shift data. The statistical methods of exploratory data analysis and discriminant analysis are used to construct two canonical coordinates-linear combinations of chemical shifts which give the statistically optimal determination of the conformation from the NMR data.

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Citations

Jun 15, 2010·Journal of Biomolecular NMR·Christian RichterHarald Schwalbe
Nov 28, 2012·Journal of Biomolecular NMR·Shawn BartonMichael F Summers
Dec 4, 2008·Journal of the American Chemical Society·James E Johnson, Charles G Hoogstraten
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Sep 26, 2009·Chemistry & Biology·Mahmoud GhanemVern L Schramm
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