Jul 10, 1981

Dependence of anaphylactic histamine release from rat mast cells on cellular energy metabolism

European Journal of Pharmacology
T Johansen


The relation between anaphylactic histamine release and the adenosine triphosphate (ATP) content of the mast cells was studied. The cells were incubated with glycolytic (2-deoxyglucose) and respiratory inhibitors (antimycin A and oligomycin) in order to decrease the ATP content of the cells prior to initiation of the release process by the antigen-antibody reaction. The secretory capacity of mast cells was less related to the cellular level of ATP at the time of activation of the release process by the antigen-antibody reaction than to the rate of cellular energy supply. Furthermore, mast cells were pretreated with 2-deoxyglucose. The release of histamine from these cells was reduced when respiratory inhibitors were added to the cell suspension 5 to 20 sec after exposure of the cells to antigen. This may indicate that the secretory process requires energy, and it seems necessary that energy should be produced as the release of histamine takes place.

  • References12
  • Citations3

Mentioned in this Paper

Histamine Measurement
Energy Supply
Mast Cell
Secretory Pathway
Energy Metabolism
August Rats
Antimycin A
Transcription Initiation

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Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.