Dependence of c-fos Expression on Amplitude of High-Frequency Spinal Cord Stimulation in a Rodent Model

Neuromodulation : Journal of the International Neuromodulation Society
Shiying LiRobert D Foreman

Abstract

Clinical high-frequency spinal cord stimulation (hfSCS) (>250 Hz) applied at subperception amplitudes reduces leg and low back pain. This study investigates, via labeling for c-fos-a marker of neural activation, whether 500 Hz hfSCS applied at amplitudes above and below the dorsal column (DC) compound action potential (CAP) threshold excites dorsal horn neurons. DC CAP thresholds in rats were determined by applying single biphasic pulses of SCS to T12 -T13 segments using pulse widths of 40 or 200 μsec via a ball electrode placed over the left DC and increasing amplitude until a short latency CAP was observed on the L5 DC and sciatic nerve. The result of this comparison allowed us to substitute sciatic nerve CAP for DC CAP. SCS at T12 -T13 was applied continuously for two hours using: sham or hfSCS at 500 Hz SCS, 40 μsec pulse width, and 50, 70, 90, or 140% CAP threshold. Spinal cord slices from T11 -L1 were immunolabeled for c-fos, and the number of c-fos-positive cells was quantified. 500 Hz hfSCS applied at 90 and 140% CAP threshold produced substantial (≥6 c-fos + neurons on average per slice per segment) c-fos expression in more segments between T11 and L1 than did sham stimulation (p < 0.025, 90% CAP; p < 0.001, 140% CAP, ...Continue Reading

References

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Jan 17, 2013·World Neurosurgery·Dirk De RidderSven Vanneste
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Jun 2, 2017·Neuromodulation : Journal of the International Neuromodulation Society·Bengt Linderoth, Robert D Foreman
Dec 9, 2017·Neuromodulation : Journal of the International Neuromodulation Society·Simon J ThomsonMichael Moffitt

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Citations

Nov 28, 2019·Neuromodulation : Journal of the International Neuromodulation Society·Jeffrey E ArleKristen W Carlson
Sep 16, 2020·Neuromodulation : Journal of the International Neuromodulation Society·Pablo AndradeGeorgios Matis
Dec 31, 2020·Neuromodulation : Journal of the International Neuromodulation Society·Qingyu YaoFengzeng Jian

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