Abstract
The characteristics of the aggregation reaction that follows allergen binding to cell surface IgE on basophils and mast cells depend on a variety of factors that include the density of IgE and the affinity of the allergen for IgE. For simple bivalent stimuli, one prediction is that the location of the optimum for aggregation is not dependent on IgE density, only the affinity for IgE. However, this behavior does not occur for stimulation with an anti-IgE antibody (Ab) during the treatment of patients with omalizumab. This study re-examined the stability of the optimum for histamine release, relative to cell surface IgE density, using the simple bivalent penicillin hapten (BPO2) or a bivalent monoclonal anti-IgE Ab. The results validated one prediction for one bivalent hapten, BPO2. Across a range of BPO-specific IgE density of 270-23,500/cell, optimal histamine release remained constant (10 nM BPO2). In contrast, across a range of approximately 6,000-110,000/cell, optimal histamine release shifted 8- to 30-fold for anti-IgE Ab. The distinguishing characteristic between the 2 bivalent stimuli was the difference in their crosslink re-equilibration. Recent modeling of histamine release suggested that the SYK-to-receptor ratio could...Continue Reading
References
Dec 1, 1971·The Journal of Experimental Medicine·A L SullivanH Metzger
Nov 1, 1972·The Journal of Clinical Investigation·E Gillespie, L M Lichtenstein
Aug 1, 1973·The Journal of Experimental Medicine·K E BeckerP M Grimley
Feb 1, 1974·Analytical Biochemistry·R P Siraganian
Feb 1, 1993·The Journal of Allergy and Clinical Immunology·D W MacGlashan
Jul 24, 1998·Science·C TorigoeH Metzger
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·Z J LiuH Metzger
Sep 10, 2004·The Journal of Allergy and Clinical Immunology·Lisa A BeckSarbjit Saini
Feb 13, 2007·The Journal of Allergy and Clinical Immunology·Elizabeth A ErwinThomas A E Platts-Mills
Mar 6, 2007·The Journal of Allergy and Clinical Immunology·Donald W MacGlashan
Jul 5, 2007·Journal of Leukocyte Biology·Donald W MacGlashan
Jul 10, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gregorio GomezLawrence B Schwartz
Mar 7, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Donald W MacGlashanDavid E Sloane
May 14, 2009·Journal of Leukocyte Biology·Susan Ishmael, Donald MacGlashan
Nov 16, 2010·IET Systems Biology·Ambarish NagByron Goldstein
Jun 19, 2012·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D MacGlashan
Apr 17, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D MacglashanU Muchhal
Feb 27, 2017·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A LewisP T Peachell
Citations
Aug 17, 2020·Current Gastroenterology Reports·Jennifer PryorSimon Keely
Apr 4, 2021·Nutrients·Kerith DuncansonNicholas J Talley
Mar 16, 2021·The Journal of Allergy and Clinical Immunology·Donald MacGlashanJohn T Schroeder