Dependence on dose of the acute effects of ethanol on liver metabolism in vivo

The Journal of Clinical Investigation
R W Guynn, J R Pieklik

Abstract

The dose dependence of the acute effects of ethanol upon liver intermediary metabolism in vivo has been demonstrated in rats. Ethanol was given i.p. in doses of 0.69, 1.7, and 3.0 g/kg in equal volumes (20 ml/kg). The liver was freeze-clamped 120 min after injection, and multiple metabolites were measured in the perchloric acid extract of the tissue. Each group showed a significantly different pattern of metabolites, redox states, and phosphorylation potentials although the rate of ethanol disappearance, at least between the two highest dose groups, was not significantly different. The mitochondrial free [NAD+]/[NADH] ratios and the cytoplasmic free [NADP+]/[NADPH] ratio were paradoxically most reduced with the lowest dose of ethanol and became progressively more oxidized with increasing dose. Once established, the differences in these ratios between the groups tended to persist with time, relatively independent of the concentration of ethanol. In a somewhat different pattern, the phosphorylation potential ([ATP]/[ADP][P1]) remained at the control level in the low-dose group but was significantly elevated in the two higher-dose groups. The results, therefore, show distinct and complicated dose-dependent patterns of intermediary...Continue Reading

References

Nov 16, 1973·Science·E Rubin, Charles S Lieber
Jan 17, 1974·The New England Journal of Medicine·E Rubin, Charles S Lieber
Dec 1, 1965·Biochemical Pharmacology·Y IsraelI Laufer
Jan 1, 1968·Advances in Enzyme Regulation·H A Krebs
Oct 1, 1969·Biochemical Pharmacology·D R Van Harken, G J Mannering
Jun 1, 1970·Biochemical Pharmacology·J M Khanna, H Kalant
Feb 1, 1972·European Journal of Biochemistry·R G ThurmanR Scholz
Oct 22, 1972·Life Sciences. Pt. 2: Biochemistry, General and Molecular Biology·N Grunnet, H I Thieden
Jan 1, 1972·Analytical Biochemistry·R W GuynnR L Veech
Jun 1, 1970·Biochemical Pharmacology·A B Makar, G J Mannering

Citations

Mar 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B E AkinsholaE Mezey
Apr 1, 1980·Biochemical Pharmacology·D J Stewart, J M Khanna
Aug 15, 1984·Biochemical Pharmacology·L Lumeng, D W Crabb
Jun 1, 1988·Biochemical Pharmacology·O A Forsander, A R Pösö
Jan 1, 1983·Pharmacology, Biochemistry, and Behavior·N W Cornell
Jul 26, 2002·Life Sciences·Enrique BaraonaCharles S Lieber
Feb 1, 1989·Metabolism: Clinical and Experimental·F ContaldoE A Nikkilä
Jan 1, 1987·Annals of the New York Academy of Sciences·C C Cunningham, P I Spach
Oct 1, 1980·Alcoholism, Clinical and Experimental Research·M R LakshmananR L Veech
Jul 1, 1977·The American Journal of Medicine·H O Heinemann
Aug 1, 1987·Archives of Biochemistry and Biophysics·L B LaBaumeR W Guynn
Jan 26, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Laura TedescoEnzo Nisoli
Mar 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B E AkinsholaE Mezey
Jan 1, 1986·Alcoholism, Clinical and Experimental Research·S AnderssonJ Sjövall
Jul 1, 1980·Alcoholism, Clinical and Experimental Research·J MørlandL Svendsen
Jan 1, 1977·Alcoholism, Clinical and Experimental Research·K E CrowR L Veech
Jan 1, 1982·Alcoholism, Clinical and Experimental Research·R W GuynnL L Shanbour
Nov 1, 1996·Alcoholism, Clinical and Experimental Research·A CahillC C Cunningham
Nov 28, 2008·American Journal of Physiology. Cell Physiology·Nirish ShahJean-Pierre Raufman
Mar 1, 1983·Acta Neurologica Scandinavica·B Melgaard
Oct 14, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ricard MasiaGary Yellen

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Ethanol
Dose-Response Relationship, Drug
Liver
NADH
NADP
Oxidation-Reduction

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.