PMID: 9425110Feb 28, 1998Paper

Dephosphorylation and deactivation of Ca2+/calmodulin-dependent protein kinase II in betaTC3-cells is mediated by Mg2+- and okadaic-acid-sensitive protein phosphatases

The Biochemical Journal
R A EasomH Bhatt

Abstract

The alpha-toxin-permeabilized betaTC3 cell has been utilized as an experimental model for the identification of protein phosphatases responsible for the dephosphorylation and deactivation of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) in situ. In this model, the elevation of Ca2+ from 0.05 to 10 microM induced the near-total conversion of CaM kinase II into a Ca2+/calmodulin-independent (autonomous) form characteristic of autophosphorylated, activated enzyme. On the removal of Ca2+, the activation state of CaM Kinase II rapidly returned to prestimulated levels. This reversal was slowed, but not prevented, by the inhibitors of protein phosphatase-1 (PP-1) and PP-2A, okadaic acid and calyculin A, and by the selective chelation of Mg2+ by the addition of EDTA. Near-complete prevention of enzyme deactivation, however, was observed in the combined presence of both okadaic acid and EDTA. Under these conditions, CaM kinase II phosphatase was more sensitive to calyculin A relative to okadaic acid, characteristic of the involvement of PP-1. CaM kinase II deactivation was not affected by FK-506, eliminating the involvement of PP-2B in this process. These data suggest that CaM kinase II dephosphorylation and deactivation i...Continue Reading

Citations

Aug 1, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·C H ChangT M Chang
Dec 10, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Nen-Chung ChangTsorng-Harn Fong
Apr 13, 2012·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Hung-Ming ChangFu-Der Mai
Dec 24, 1998·Molecular and Cellular Endocrinology·G Piwien-Pilipuk, M D Galigniana
Jun 12, 2003·Developmental Biology·Styliani MarkoulakiTom Ducibella
Jul 10, 2004·Developmental Biology·Styliani MarkoulakiTom Ducibella

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