Dephosphorylation of sodium caseinate, enzymatically hydrolyzed casein and casein phosphopeptides by intestinal alkaline phosphatase: implications for iron availability

The Journal of Nutritional Biochemistry
A C. YeungDennis D Miller

Abstract

Clusters of phosphoserine residues in casein bind iron with high affinity. Casein inhibits iron absorption in humans but partial hydrolysis of casein prior to ingestion diminishes this inhibition. The objective of this study was to test two hypotheses: 1. Partial hydrolysis of the peptide bonds in casein exposes phosphoserine residues to attack by intestinal alkaline phosphatase (IAP). 2. Hydrolysis of the phospho-ester linkage in phosphoserine residues in casein by IAP releases bound iron or inhibits iron chelation, thereby allowing its absorption. Test of hypothesis 1: Suspensions of sodium caseinate (SC), enzymatically hydrolyzed casein (EHC), and casein phosphopeptides (CPP) were subjected to an in vitro pepsin/pancreatin digestion and subsequently incubated in the presence of calf IAP. The rate of release of inorganic phosphate was measured with the following results (expressed as &mgr;mol phosphate released/unit of IAP/min): 0.081, 0.104, 0.139 for SC, EHC, and CPP, respectively. These results are consistent with hypothesis 1. Test of hypothesis 2: (59)Fe-citrate or (59)Fe-citrate + CPP in minimum essential media were spiked with a Na(2)WO(4) solution or water (Na(2)WO(4) is a known inhibitor of IAP) and placed on Caco-2 ...Continue Reading

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Citations

Aug 9, 2003·Journal of Physiology and Biochemistry·F Pérez-LlamasS Zamora
Sep 29, 2005·Pediatric Research·Ida B KibangouDominique L Bouglé
Jul 5, 2005·The Journal of Nutritional Biochemistry·Bertille Ani-KibangouDominique Bouglé
Jul 15, 2015·Journal of Agricultural and Food Chemistry·María José García-NebotSaïd Bouhallab
Jul 22, 2008·Biochemical and Biophysical Research Communications·Raghu PullakhandamTippeswamy Gowda Thippande
Jun 19, 2007·Biochimica Et Biophysica Acta·Kevin M MilleaScott J Berger
Jan 6, 2004·The Journal of Nutrition·Paz EtcheverryRaymond P Glahn
Mar 18, 2009·Journal of Agricultural and Food Chemistry·Indah EpriliatiMike Gidley
Nov 14, 2002·Journal of Agricultural and Food Chemistry·Saíd BouhallabDominique Bouglé
Nov 9, 2007·Journal of Agricultural and Food Chemistry·Konstantina ArgyriMaria Kapsokefalou

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