PMID: 7520171Aug 16, 1994Paper

Dephosphorylation of sperm midpiece antigens initiates aster formation in rabbit oocytes

Proceedings of the National Academy of Sciences of the United States of America
C Pinto-CorreiaJ M Robl

Abstract

During fertilization in most mammals, the penetrating sperm organizes an aster of microtubules. We have investigated the mechanisms underlying this function of the sperm by a series of experiments based on microinjection of isolated sperm midpieces into unfertilized oocytes. These midpieces contain antigens recognized by the MPM-2 antibody. These antigens, which are absent from the rest of the tail fraction, correspond to three phosphorylated polypeptides of 77, 81, and 85 kDa. Dephosphorylation with alkaline phosphatase abolishes antigenicity on blots and in whole sperm. Reactivity to the antibody disappears between 1 and 3 hr after calcium stimulation of oocytes, following the decline in H1 kinase activity and coincident with aster formation. In unactivated oocytes, no aster forms and the antigen remains unchanged. MPM-2 treatment of midpieces prior to injection blocks their ability to form asters in oocytes activated by calcium stimulation. The epitope also disappears in 6-methyl-aminopurine-treated oocytes, implying that maintenance of the phosphorylated state requires kinase activity. A result that confirms this view is that sperm midpieces dephosphorylated by alkaline phosphatase can be rephosphorylated after injection in...Continue Reading

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Citations

Nov 20, 1998·Molecular Reproduction and Development·N H KimK S Chung
Feb 18, 2011·The Journal of Reproduction and Development·Hiromasa HaraShinichi Hochi
Nov 5, 2011·The Journal of Reproduction and Development·Shinichi HochiMasumi Hirabayashi
Jul 23, 2008·Molecular Reproduction and Development·Masahito TachibanaKunihiro Okamura
May 14, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Shinichi Hochi
Jul 28, 2005·Reproductive Medicine and Biology·Yukihiro TeradaKunihiro Okamura
Oct 22, 2019·Frontiers in Cell and Developmental Biology·Tomer Avidor-ReissPuneet Sindhwani

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