Depicting the spatial distribution of proteins in human tumor tissue combining SELDI and MALDI imaging and immunohistochemistry.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Liane WehderFerdinand von Eggeling

Abstract

Carcinoma tissue consists of not only tumor cells but also fibroblasts, endothelial cells or vascular structures, and inflammatory cells forming the supportive tumor stroma. Therefore, the spatial distribution of proteins that promote growth and proliferation in these complex functional units is of high interest. Matrix-assisted laser desorption/ionization imaging mass spectrometry is a newly developed technique that generates spatially resolved profiles of protein signals directly from thin tissue sections. Surface-enhanced laser desorption/ionization mass spectrometry (MS)combined with tissue microdissection allows analysis of defined parts of the tissue with a higher sensitivity and a broader mass range. Nevertheless, both MS-based techniques have a limited spatial resolution. IHC is a technique that allows a resolution down to the subcellular level. However, the detection and measurement of a specific protein expression level is possible only by semiquantitative methods. Moreover, prior knowledge about the identity of the proteins of interest is necessary. In this study, we combined all three techniques to gain highest spatial resolution, sensitivity, and quantitative information. We used frozen tissue from head and neck tu...Continue Reading

References

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Citations

Sep 8, 2012·Journal of Cancer Research and Clinical Oncology·Theodore AlexandrovFerdinand von Eggeling
Nov 10, 2012·Analytical and Bioanalytical Chemistry·Bernd EnthalerJens-Peter Vietzke
Apr 26, 2011·Journal of Neuroscience Methods·Marlène MaitreJean-Michel Revest
Nov 7, 2014·Journal of the American Society for Mass Spectrometry·Lukas KrasnyAnna C Crecelius

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Methods Mentioned

BETA
surgical resection
protein chip
chips
laser-capture microdissection
biopsies
imaging techniques

Software Mentioned

flexImaging
SELDI

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