PMID: 8938256Feb 9, 1996Paper

Depleted internal store-activated Ca2+ entry can trigger neurotransmitter release in bovine chromaffin cells

Neuroscience Letters
D A PowisK J O'Brien

Abstract

A potential role of the intracellular Ca2+ stores in modulating catecholamine release has been investigated in bovine chromaffin cells maintained in tissue culture. Pharmacological depletion of the stores with a combination of caffeine, histamine and thapsigargin in Ca2+-free media resulted in a significantly greater release of catecholamines on re-exposure to Ca2+-containing media compared with that from non-store depleted cells. The increase in catecholamine release was prevented by intracellular BAPTA indicating that the increase was caused by a rise in Ca2+. Measurement of intracellular free Ca2+ concentration with the fluorescent indicator, fura-2, over the same time-course as the catecholamine release experiments showed that upon restoration of external Ca2+ there was an immediate, substantial and maintained increase in cytosolic Ca2+. It is most probable that the increase in catecholamine release was a consequence of an increase in Ca2+ influx triggered by prior depletion of the internal Ca2+ stores. However, the data suggest that capacitative Ca2+ entry is poorly linked to catecholamine release; although Ca2+ entry on restoration of external Ca2+ was immediate and substantial, the increase in catecholamine release, alth...Continue Reading

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Citations

Apr 2, 2003·Pharmacology & Therapeutics·Philip D Marley
Dec 22, 1999·British Journal of Pharmacology·P J BalesP D Marley
May 25, 2002·The European Journal of Neuroscience·Sacha McKenzie, Philip D Marley
Nov 20, 2002·Annals of the New York Academy of Sciences·David A Powis, Mariann Zerbes
Aug 4, 2006·Journal of Neurophysiology·Babak A KachoeiNeil S Magoski
Jan 3, 2012·Cell Calcium·Antonio G GarcíaJavier García-Sancho
Mar 29, 2000·The Journal of Biological Chemistry·M L MundorfR M Wightman
Dec 3, 1999·Journal of Neurochemistry·M L MundorfR M Wightman

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