Depleting Macrophages In Vivo with Clodronate-Liposomes

Methods in Molecular Biology
Stéphanie G Moreno

Abstract

In order to decipher the role of macrophages in vivo, it could be necessary to establish a model of macrophage depletion in the whole animal. One method to obtain animal models efficiently depleted in macrophages in different tissues (bone marrow, spleen, liver, lungs, brain, gut, peritoneal cavity, lymph nodes/vessels) and blood is the use of a clodronate-liposome solution.Here, we describe the protocol used to deplete efficiently macrophages in mouse bone marrow.

Citations

Aug 29, 2020·Advanced Materials·Yuqiong XiaXiaoyuan Chen
Jul 16, 2020·Proceedings of the National Academy of Sciences of the United States of America·Anvay UkidveSamir Mitragotri
Dec 1, 2020·International Journal of Radiation Biology·Wen JuLingyu Zeng
Mar 26, 2021·Frontiers in Immunology·Chunye ZhangAaron C Ericsson
Mar 15, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Junmin FuTiantai Zhang
May 4, 2021·Frontiers in Cellular and Infection Microbiology·Yishan ZhangHong Zhang

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